Rabbit DERL1 Polyclonal Antibody | anti-DERL1 antibody

DERL1 Antibody (C-term)

Cat. Num. AAA287090

• Gene Names: DERL1; DER1; DER-1
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DERL1, Antibody; DERL1 Antibody (C-term); Derlin-1; Degradation in endoplasmic reticulum protein 1; DERtrin-1; Der1-like protein 1; DERL1; DER1; anti-DERL1 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DERL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human DERL1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 224-251
• Sequence Length: 231

• Applicable Applications for anti-DERL1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(DERL1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DERL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of DERL1 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). DERL1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-DERL1 antibody

Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. DERL1 may act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. It may mediate the interaction between VCP and the degradation substrate. In case of infection by cytomegaloviruses, it plays a central role in the export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Also participates in the degradation process of misfolded cytomegalovirus US2 protein.

Product Categories/Family for anti-DERL1 antibody

Signal Transduction; Amyotrophic Lateral Sclerosis (ALS)

References

Oda Y., et.al., J. Cell Biol. 172:383-393(2006). Ye Y., et.al., Proc. Natl. Acad. Sci. U.S.A. 102:14132-14138(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 197927278
• NCBI GeneID: 79139
• NCBI Accession #: NP_001128143.1; NP_077271.1
• NCBI GenBank Nucleotide #: NM_001134671.2
• UniProt Accession #: Q9BUN8; B3KW41; E9PH19
• Molecular Weight: 28801
• NCBI Official Full Name: derlin-1 isoform b
• NCBI Official Synonym Full Names: derlin 1
• NCBI Official Symbol: DERL1
• NCBI Official Synonym Symbols: DER1; DER-1
• NCBI Protein Information: derlin-1
• UniProt Protein Name: Derlin-1
• UniProt Gene Name: DERL1
• UniProt Synonym Gene Names: DER1; DERtrin-1
• UniProt Entry Name: DERL1_HUMAN