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IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-7.JPGIHC(P): Rat Cardiac Muscle Tissue)
Rabbit Desmin Polyclonal Antibody | anti-DES antibody
Anti-Desmin Antibody
Gene Names
DES; CSM1; CSM2; LGMD2R
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Desmin; Polyclonal Antibody; Anti-Desmin Antibody; desmin; CMD1I antibody; CSM1 antibody; CSM2 antibody; DES antibody; DESM_HUMAN antibody; Desmin antibody; FLJ12025 antibody; FLJ39719 antibody; FLJ41013 antibody; FLJ41793 antibody; Intermediate filament protein antibody; OTTHUMP00000064865 antibody; anti-DES antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
470
Applicable Applications for anti-DES antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin
Application Notes
WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat
IHC-P: Concentation: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Mouse, Rat
WB: The detection limit for Desmin is approximately 0.25ng/lane under reducing conditions.
Tested Species: In house tested species with positive results.
By Heat: Boilin gthe paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested. Optimal dilutions should be determined by end users.
IHC-P: Concentation: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Mouse, Rat
WB: The detection limit for Desmin is approximately 0.25ng/lane under reducing conditions.
Tested Species: In house tested species with positive results.
By Heat: Boilin gthe paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen
E Coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequences identity with both mouse and rat Desmin.
Cross Reactivity
No cross reactivity with other proteins.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-7.JPGIHC(P): Rat Cardiac Muscle Tissue)
IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-7.JPGIHC(P): Rat Cardiac Muscle Tissue)
IHC (Immunohistchemistry)
(Anti-Desmin Picoband antibody, AAA11607-6.JPGIHC(P): Mouse Skeletal Muscle Tissue)
IHC (Immunohistchemistry)
(Anti-Desmin Picoband antibody, AAA11607-6.JPGIHC(P): Mouse Skeletal Muscle Tissue)
IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-5.JPGIHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-5.JPGIHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-4.JPGIHC(F): Mouse Cardiac Muscle Tissue)
IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-4.JPGIHC(F): Mouse Cardiac Muscle Tissue)
IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-3.JPGIHC(F): Rat Cardiac Muscle Tissue)
IHC (Immunohistochemistry)
(Anti-Desmin Picoband antibody, AAA11607-3.JPGIHC(F): Rat Cardiac Muscle Tissue)
Application Data
(Anti-Desmin Picoband antibody, AAA11607-2.jpgAll lanes: Anti Desmin (AAA11607) at 0.5ug/mlLane 1: Rat Skeletal Muscle Tissue Lysate at 50ugLane 2: Rat Cardiac MuscleTissue Lysate at 50ugLane 3: Mouse Skeletal Muscle Tissue Lysate at 50ugLane 4: Mouse Cardiac MuscleTissue Lysate at 50ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: HT1080 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: HEPA Whole Cell Lysate at 40ugLane 9: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 53KD)
Application Data
(Anti-Desmin Picoband antibody, AAA11607-2.jpgAll lanes: Anti Desmin (AAA11607) at 0.5ug/mlLane 1: Rat Skeletal Muscle Tissue Lysate at 50ugLane 2: Rat Cardiac MuscleTissue Lysate at 50ugLane 3: Mouse Skeletal Muscle Tissue Lysate at 50ugLane 4: Mouse Cardiac MuscleTissue Lysate at 50ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: HT1080 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: HEPA Whole Cell Lysate at 40ugLane 9: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 53KD)
Application Data
(Anti-Desmin Picoband antibody, AAA11607-1.jpgAll lanes: Anti Desmin (AAA11607) at 0.5ug/mlWB: Recombinant Human Desmin Protein 0.5ngPredicted bind size: 36KDObserved bind size: 36KD)
Application Data
(Anti-Desmin Picoband antibody, AAA11607-1.jpgAll lanes: Anti Desmin (AAA11607) at 0.5ug/mlWB: Recombinant Human Desmin Protein 0.5ngPredicted bind size: 36KDObserved bind size: 36KD)
Related Product Information for anti-DES antibody
Description: Rabbit IgG polyclonal antibody for Desmin(DES) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.
Background: DES, also called desmin, is a protein that in humans is encoded by the DES gene, and this gene is mapped to 2q35. DES is the muscle-specific member of the intermediate filament (IF) protein family. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. DES is very important in muscle cell architecture and structure since it connects many components of the cytoplasm. It may be also play an important role in mitochondria function. What's more, DES provides attachments between the terminal Z disc and membrane-associated proteins to form a force-transmitting system that parallels the thin filaments at myotendinous junctions.
Background: DES, also called desmin, is a protein that in humans is encoded by the DES gene, and this gene is mapped to 2q35. DES is the muscle-specific member of the intermediate filament (IF) protein family. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. DES is very important in muscle cell architecture and structure since it connects many components of the cytoplasm. It may be also play an important role in mitochondria function. What's more, DES provides attachments between the terminal Z disc and membrane-associated proteins to form a force-transmitting system that parallels the thin filaments at myotendinous junctions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Desmin
NCBI Official Synonym Full Names
desmin
NCBI Official Symbol
DES
NCBI Official Synonym Symbols
CSM1; CSM2; LGMD2R
NCBI Protein Information
desmin; mutant desmin p.K241E; intermediate filament protein
UniProt Protein Name
Desmin
UniProt Gene Name
DES
UniProt Entry Name
DESM_HUMAN
Similar Products
Product Notes
The DES des (Catalog #AAA11607) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Desmin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Desmin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin. WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat IHC-P: Concentation: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Mouse, Rat WB: The detection limit for Desmin is approximately 0.25ng/lane under reducing conditions. Tested Species: In house tested species with positive results. By Heat: Boilin gthe paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the DES des for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Desmin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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