IHC (Immunohistochemisry)
(Detection of human DFF45/DFFA by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DFF45/DFFA (Cat. No. AAA213929) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.)
Rabbit anti-Human DFF45/DFFA Polyclonal Antibody | anti-DFF45/DFFA antibody
Rabbit anti-DFF45/DFFA IHC Antibody, Affinity Purified
Gene Names
DFFA; DFF1; ICAD; DFF-45
Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
DFF45/DFFA, Antibody; Rabbit anti-DFF45/DFFA IHC Antibody, Affinity Purified; DNA fragmentation factor 45 kDa subunit; ICAD; inhibitor of CAD; DNA fragmentation factor subunit alpha; DFF45; DFF1; DNA fragmentation factor, 45kDa, alpha polypeptide; DFF-45; anti-DFF45/DFFA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for anti-DFF45/DFFA antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Predicted Reactivity
Orangutan, Chimpanzee, Northern white-cheeked gibbon
Immunogen
Between 281 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IHC (Immunohistochemisry)
(Detection of human DFF45/DFFA by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DFF45/DFFA (Cat. No. AAA213929) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.)
IHC (Immunohistochemisry)
(Detection of human DFF45/DFFA by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DFF45/DFFA (Cat. No. AAA213929) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.)
IHC (Immunohiostchemistry)
(Detection of human DFF45/DFFA by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DFF45/DFFA (Cat. No. AAA213929) used at a dilution of 1:100. Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human DFF45/DFFA by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DFF45/DFFA (Cat. No. AAA213929) used at a dilution of 1:100. Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DFF45/DFFA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DFF45/DFFA (Cat. No. AAA213929) used at a dilution of 1:500. Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DFF45/DFFA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DFF45/DFFA (Cat. No. AAA213929) used at a dilution of 1:500. Detection: DAB)
Related Product Information for anti-DFF45/DFFA antibody
DNA fragmentation factor (DFF45/DFFA) is a component of DFF, the principle DNA endonuclease responsible for nucleosomal DNA cleavage during apoptosis. In the DFF complex, DFF45/DFFA heterodimerizes with DFF40 and is a substrate of the apoptotic effector, caspase 3. Initiation of the cellular apoptotic program results in the activation of caspase 3 and the cleavage and release of DFF45 from its heterodimeric partner, DFF40. The release of DFF40 from the inhibitory activity of DFF45 allows DFF40 to function as a DNA endonuclease to promote chromosomal DNA fragmentation. A 35 kDa splice variant of DFF45, DFF35, also exists and functions as a chaperone and alternative heterodimeric partner for DFF40.
Product Categories/Family for anti-DFF45/DFFA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,411 Da
NCBI Official Full Name
Homo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 2, mRNA
NCBI Official Synonym Full Names
DNA fragmentation factor, 45kDa, alpha polypeptide
NCBI Official Symbol
DFFA
NCBI Official Synonym Symbols
DFF1; ICAD; DFF-45
NCBI Protein Information
DNA fragmentation factor subunit alpha; DFF45; DNA fragmentation factor 45 kDa subunit; inhibitor of CAD
UniProt Protein Name
DNA fragmentation factor subunit alpha
UniProt Gene Name
DFFA
UniProt Synonym Gene Names
DFF1; DFF45; DFF-45; ICAD
UniProt Entry Name
DFFA_HUMAN
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Product Notes
The DFF45/DFFA dffa (Catalog #AAA213929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DFF45/DFFA IHC Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DFF45/DFFA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the DFF45/DFFA dffa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DFF45/DFFA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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