FCM/FACS (Flow Cytometry)
(DGCR8 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit DGCR8 Polyclonal Antibody | anti-DGCR8 antibody
DGCR8 Antibody (Center)
Gene Names
DGCR8; Gy1; pasha; DGCRK6; C22orf12
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DGCR8, Antibody; DGCR8 Antibody (Center); Microprocessor complex subunit DGCR8; DiGeorge syndrome critical region 8; DGCR8; C22orf12; DGCRK6; anti-DGCR8 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DGCR8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-466 amino acids from the Central region of human DGCR8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.396 (varies by lot)
Sequence Positions
439-466
Sequence Length
740
Applicable Applications for anti-DGCR8 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(DGCR8 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DGCR8 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of DGCR8 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). DGCR8 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of DGCR8 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). DGCR8 (arrow) was detected using the purified Pab.)
Related Product Information for anti-DGCR8 antibody
MicroRNAs (miRNA) are a recently discovered family of short non-protein-coding RNAs that negatively regulate gene expression. Recent studies of miRNAs highlight a requirement for cell viability. Post-transcriptional silencing of target genes by miRNAs occurs either by targeting specific cleavage of homologous mRNAs, or by targeting specific inhibition of protein synthesis.
References
Clague,J., et.al., Mol. Carcinog. 49 (2), 183-189 (2010)
Shenoy,A. et.al., PLoS ONE 4 (9), E6971 (2009)
Shenoy,A. et.al., PLoS ONE 4 (9), E6971 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86045
NCBI Official Full Name
microprocessor complex subunit DGCR8 isoform 2
NCBI Official Synonym Full Names
DGCR8 microprocessor complex subunit
NCBI Official Symbol
DGCR8
NCBI Official Synonym Symbols
Gy1; pasha; DGCRK6; C22orf12
NCBI Protein Information
microprocessor complex subunit DGCR8
UniProt Protein Name
Microprocessor complex subunit DGCR8
UniProt Gene Name
DGCR8
UniProt Synonym Gene Names
C22orf12; DGCRK6
UniProt Entry Name
DGCR8_HUMAN
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Product Notes
The DGCR8 dgcr8 (Catalog #AAA284529) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 439-466. The DGCR8 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's DGCR8 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DGCR8 dgcr8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGCR8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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