Rabbit anti-Human, Mouse DHCR7 Polyclonal Antibody | anti-DHCR7 antibody

DHCR7 Antibody (C-term)

Cat. Num. AAA28753

• Gene Names: DHCR7; SLOS
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: DHCR7, Antibody; DHCR7 Antibody (C-term); 7-dehydrocholesterol reductase; 7-DHC reductase; Putative sterol reductase SR-2; Sterol Delta(7)-reductase; DHCR7; D7SR; anti-DHCR7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This DHCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-463 amino acids from the C-terminal region of human DHCR7.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 437-463
• Sequence Length: 475

• Applicable Applications for anti-DHCR7 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
• Application Notes: IF~~ 1:10~50; WB~~1:2000; IHC-P~~1:50~100
• Function: 7-dehydrocholesterol reductase of the cholesterol biosynthetic pathway reducing the C7-C8 double bond of cholesta-5,7- dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien- 3beta-ol, two intermediates in that pathway.
• Cellular Location: Endoplasmic reticulum membrane; Multi-passmembrane protein
• Tissue Location: Widely expressed. Most abundant in adrenal gland, liver, testis,and brain.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-DHCR7 Antibody (C-term) at 1:2000 dilution Lane 1: Caco2 whole cell lysateLane 2: mouse testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (please inquire) at 1/15000 dilution.Observed band size : 55kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(DHCR7 Antibody (C-term) (AAA28753) western blot analysis in mouse liver tissue lysates (35ug/lane). This demonstrates the DHCR7 antibody detected the DHCR7 protein(arrow).)

WB (Western Blot)

(All lanes : Anti-DHCR7 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Li-7 whole cell lysate Lane 3: U-87MG whole cell lysate Lane 4: mouse testis lysate Lysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohistochemistry)

(DHCR7 Antibody (C-term) (AAA28753) Immunohistochemistry analysis informalin fixed and paraffin embedded humantestis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DHCR7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of DHCR7 Antibody (C-term) (AAA28753) with MCF-7 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG(green).DAPI was used to stain the cell nuclear(blue).)

IF (Immunofluorescence)

(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelialadeno carcinoma cell line) cells labeling Pdx1with AAA28753 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight®554 Phalloidin at 1/100 dilution(red). The nuclear counter stain is DAPI (blue).)

Product Categories/Family for anti-DHCR7 antibody

Cancer; Cardiovascular; Metabolism; Signal transduction

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Koo, G., et al. Am. J. Med. Genet. A 152A (8), 2094-2098 (2010) :
Wang, T.J., et al. Lancet 376(9736):180-188(2010)
Ahn, J., et al. Hum. Mol. Genet. 19(13):2739-2745(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 255308875
• NCBI GeneID: 1717
• NCBI Accession #: NP_001157289.1; NP_001351.2
• NCBI GenBank Nucleotide #: NM_001163817.1
• UniProt Accession #: Q9UBM7; O60492; O60717; B2R6Z2
• NCBI Official Full Name: 7-dehydrocholesterol reductase
• NCBI Official Synonym Full Names: 7-dehydrocholesterol reductase
• NCBI Official Symbol: DHCR7
• NCBI Official Synonym Symbols: SLOS
• NCBI Protein Information: 7-dehydrocholesterol reductase
• UniProt Protein Name: 7-dehydrocholesterol reductase
• UniProt Gene Name: DHCR7
• UniProt Synonym Gene Names: D7SR; 7-DHC reductase
• UniProt Entry Name: DHCR7_HUMAN