WB (Western Blot)
(Detection of human DHX15 by western blot and immunoprecipitation. Samples: Whole cell lysate (CL; 60 ug for WB, 1 mg for IP) from HEK293T cells. Antibodies: Affinity purified rabbit anti-DHX15 antibody BL1628 (Cat. No. AAA210925) used at 0.1 ug/ml for WB and at 1 ug/mg lysate for IP. Immunoprecipitated DHX15 was blotted using affinity purified rabbit anti-DHX15 antibody BL1627 (Cat. No. at 0.1 ug/ml. BL1627 was also used unsuccessfully for IP (1 ug/mg lysate). Detection: Chemiluminescence with an exposure time of 15 seconds.)
Rabbit anti-Human DHX15 Polyclonal Antibody | anti-DHX15 antibody
Rabbit anti-DHX15 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DHX15, Antibody; Rabbit anti-DHX15 Antibody, Affinity Purified; PRP43; RNA helicase 2; putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; PRPF43; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; HRH2; DEAH box protein 15; DEAH (Asp-Glu-Ala-His) box helicase 15; DEAD/H box-15; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15; DDX15; DBP1; ATP-dependent RNA helicase #46; DEAH (Asp-Glu-Ala-His) box polypeptide 15; PrPp43p; Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; DEAH-box helicase 15; anti-DHX15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DHX15 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Orangutan
Immunogen
Between 745 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DHX15 by western blot and immunoprecipitation. Samples: Whole cell lysate (CL; 60 ug for WB, 1 mg for IP) from HEK293T cells. Antibodies: Affinity purified rabbit anti-DHX15 antibody BL1628 (Cat. No. AAA210925) used at 0.1 ug/ml for WB and at 1 ug/mg lysate for IP. Immunoprecipitated DHX15 was blotted using affinity purified rabbit anti-DHX15 antibody BL1627 (Cat. No. at 0.1 ug/ml. BL1627 was also used unsuccessfully for IP (1 ug/mg lysate). Detection: Chemiluminescence with an exposure time of 15 seconds.)
WB (Western Blot)
(Detection of human DHX15 by western blot and immunoprecipitation. Samples: Whole cell lysate (CL; 60 ug for WB, 1 mg for IP) from HEK293T cells. Antibodies: Affinity purified rabbit anti-DHX15 antibody BL1628 (Cat. No. AAA210925) used at 0.1 ug/ml for WB and at 1 ug/mg lysate for IP. Immunoprecipitated DHX15 was blotted using affinity purified rabbit anti-DHX15 antibody BL1627 (Cat. No. at 0.1 ug/ml. BL1627 was also used unsuccessfully for IP (1 ug/mg lysate). Detection: Chemiluminescence with an exposure time of 15 seconds.)
WB (Western Blot)
(Detection of human DHX15 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody AAA210925 (lot AAA210925-1) used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human DHX15 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody AAA210925 (lot AAA210925-1) used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-DHX15 antibody
DHX15 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX15 is the human ortholog of the yeast Prp43 protein. DHX15 has been demonstrated to function in ribosome biogenesis and pre-mRNA splicing.
Product Categories/Family for anti-DHX15 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The DHX15 dhx15 (Catalog #AAA210925) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DHX15 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DHX15 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DHX15 dhx15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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