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product-image-AAA291550_WB10.jpg WB (Western Blot) (Sample:Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-DHX36 (bs-14319R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kD)

Rabbit anti-Rat, Cow DHX36 Polyclonal Antibody | anti-DHX36 antibody

DHX36 Polyclonal Antibody

Gene Names
DHX36; G4R1; RHAU; DDX36; MLEL1
Reactivity
Rat, Cow
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DHX36, Antibody; DHX36 Polyclonal Antibody; ATP-dependent DNA/RNA helicase DHX36; 3.6.4.12; 3.6.4.13; DEAD/H box polypeptide 36; DEAH-box protein 36; G4-resolvase-1; G4R1; MLE-like protein 1; RNA helicase associated with AU-rich element protein; DHX36; anti-DHX36 antibody
Ordering
Host
Rabbit
Reactivity
Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-DHX36 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Sample:Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-DHX36 (bs-14319R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kD)

product-image-AAA291550_WB10.jpg WB (Western Blot) (Sample:Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-DHX36 (bs-14319R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kD)

IHC (Immunohistochemisry)

(Dilution: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

product-image-AAA291550_IHC11.jpg IHC (Immunohistochemisry) (Dilution: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

IHC (Immunohiostchemistry)

(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

product-image-AAA291550_IHC13.jpg IHC (Immunohiostchemistry) (Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

IHC (Immunohistochemistry)

(Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

product-image-AAA291550_IHC15.jpg IHC (Immunohistochemistry) (Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
1008
NCBI Official Full Name
ATP-dependent RNA helicase DHX36
NCBI Official Synonym Full Names
DEAH (Asp-Glu-Ala-His) box polypeptide 36
NCBI Official Symbol
DHX36
NCBI Official Synonym Symbols
G4R1; RHAU; DDX36; MLEL1
NCBI Protein Information
ATP-dependent RNA helicase DHX36; G4 resolvase-1; G4-resolvase 1; MLE-like protein 1; DEAH box protein 36; probable ATP-dependent RNA helicase DHX36; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36; RNA helicase associated with AU-rich element ARE
UniProt Protein Name
ATP-dependent RNA helicase DHX36
UniProt Gene Name
DHX36
UniProt Synonym Gene Names
DDX36; KIAA1488; MLEL1; RHAU; G4R1
UniProt Entry Name
DHX36_HUMAN

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Product Notes

The DHX36 dhx36 (Catalog #AAA291550) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHX36 Polyclonal Antibody reacts with Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's DHX36 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DHX36 dhx36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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