WB (Western Blot)
(Detection of human and mouse DHX38 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DHX38 antibody BL3386 (Cat. No. AAA211185) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). DHX38 was also immunoprecipitated by rabbit anti-DHX38 antibody BL3385 (Cat. No. which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
Rabbit anti-Human, Mouse DHX38 Polyclonal Antibody | anti-DHX38 antibody
Rabbit anti-DHX38 Antibody, Affinity Purified
Gene Names
DHX38; DDX38; PRP16; PRPF16
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DHX38, Antibody; Rabbit anti-DHX38 Antibody, Affinity Purified; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38; PRP16 homolog of S.cerevisiae; PRP16; PRPF16; pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16; DDX38; ATP-dependent RNA helicase DHX38; DEAH box protein 38; DEAH (Asp-Glu-Ala-His) box polypeptide 38; Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16; DEAH-box helicase 38; anti-DHX38 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DHX38 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 1177 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse DHX38 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DHX38 antibody BL3386 (Cat. No. AAA211185) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). DHX38 was also immunoprecipitated by rabbit anti-DHX38 antibody BL3385 (Cat. No. which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human and mouse DHX38 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DHX38 antibody BL3386 (Cat. No. AAA211185) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). DHX38 was also immunoprecipitated by rabbit anti-DHX38 antibody BL3385 (Cat. No. which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human DHX38 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DHX38 (Cat. No. AAA211185 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DHX38 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DHX38 (Cat. No. AAA211185 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-DHX38 antibody
DHX38 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX38 is the human ortholog of the yeast Prp16 protein. DHX38 has been shown to be required for catalytic step II of pre-mRNA splicing.
Product Categories/Family for anti-DHX38 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,743 Da
NCBI Official Full Name
Homo sapiens DEAH (Asp-Glu-Ala-His) box polypeptide 38 (DHX38), mRNA
NCBI Official Synonym Full Names
DEAH (Asp-Glu-Ala-His) box polypeptide 38
NCBI Official Symbol
DHX38
NCBI Official Synonym Symbols
DDX38; PRP16; PRPF16
NCBI Protein Information
pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16; ATP-dependent RNA helicase DHX38; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38; DEAH box protein 38; PRP16 homolog of S.cerevisiae; pre-mRNA splicing factor ATP-dependent RNA helicase PRP16
UniProt Protein Name
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
UniProt Gene Name
DHX38
UniProt Synonym Gene Names
DDX38; KIAA0224; PRP16
UniProt Entry Name
PRP16_HUMAN
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Product Notes
The DHX38 dhx38 (Catalog #AAA211185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DHX38 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHX38 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DHX38 dhx38 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX38, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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