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WB (Western Blot)
(Detection of human DHX8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX8 antibody AAA211060 (lot AAA211060-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse DHX8 Polyclonal Antibody | anti-DHX8 antibody
Rabbit anti-DHX8 Antibody, Affinity Purified
Gene Names
DHX8; DDX8; HRH1; PRP22; PRPF22
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DHX8, Antibody; Rabbit anti-DHX8 Antibody, Affinity Purified; DEAH (Asp-Glu-Ala-His) box polypeptide 8; PRPF22; PRP22; HRH1; RNA helicase HRH1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase); DDX8; ATP-dependent RNA helicase DHX8; Dhr2; DEAH box protein 8; DEAH-box helicase 8; anti-DHX8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DHX8 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 325 and 375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DHX8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX8 antibody AAA211060 (lot AAA211060-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human DHX8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX8 antibody AAA211060 (lot AAA211060-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DHX8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX8 antibody AAA211060 (lot AAA211060-2) used for IP at 3 ug per reaction. DHX8 was also immunoprecipitated by rabbit anti-DHX8 antibody For blotting immunoprecipitated DHX8, AAA211060 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human DHX8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX8 antibody AAA211060 (lot AAA211060-2) used for IP at 3 ug per reaction. DHX8 was also immunoprecipitated by rabbit anti-DHX8 antibody For blotting immunoprecipitated DHX8, AAA211060 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohiostchemistry)
(Detection of human DHX8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody (AAA211060 lot 2) used at 1:1000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohiostchemistry)
(Detection of human DHX8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody (AAA211060 lot 2) used at 1:1000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of mouse DHX8 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody (AAA211060 lot 2) used at 1:1000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of mouse DHX8 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody (AAA211060 lot 2) used at 1:1000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Related Product Information for anti-DHX8 antibody
DHX8 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX8 is the human ortholog of the yeast Prp22 protein. DHX8 has been demonstrated to function in the release of RNA from the splicesome and the nuclear export of spliced mRNA.
Product Categories/Family for anti-DHX8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
139,315 Da
NCBI Official Full Name
ATP-dependent RNA helicase DHX8
NCBI Official Synonym Full Names
DEAH (Asp-Glu-Ala-His) box polypeptide 8
NCBI Official Symbol
DHX8
NCBI Official Synonym Symbols
DDX8; HRH1; PRP22; PRPF22
NCBI Protein Information
ATP-dependent RNA helicase DHX8; RNA helicase HRH1; DEAH box protein 8; DEAH-box protein 8; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)
UniProt Protein Name
ATP-dependent RNA helicase DHX8
UniProt Gene Name
DHX8
UniProt Synonym Gene Names
DDX8
UniProt Entry Name
DHX8_HUMAN
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Product Notes
The DHX8 dhx8 (Catalog #AAA211060) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DHX8 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHX8 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DHX8 dhx8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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