WB (Western Blot)
(Detection of human DHX9 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody AAA211182 (lot AAA211182-4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human DHX9 Polyclonal Antibody | anti-DHX9 antibody
Rabbit anti-DHX9 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DHX9, Antibody; Rabbit anti-DHX9 Antibody, Affinity Purified; NDHII; RNA helicase A; nuclear DNA helicase II; NDH2; NDH II; LKP; leukophysin; DEAH-box helicase 9; DEAH box protein 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box helicase 9; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DDX9; ATP-dependent RNA helicase A; RHA; DExH-box helicase 9; anti-DHX9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DHX9 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 1220 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DHX9 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody AAA211182 (lot AAA211182-4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human DHX9 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody AAA211182 (lot AAA211182-4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human DHX9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX9 antibody (AAA211182-4) used for IP at 6 ug per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody (AAA211182 lot 3). For blotting immunoprecipitated DHX9, AAA211182 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human DHX9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX9 antibody (AAA211182-4) used for IP at 6 ug per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody (AAA211182 lot 3). For blotting immunoprecipitated DHX9, AAA211182 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human DHX9 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DHX9 (Cat. No. AAA211182 Lot4) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DHX9 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DHX9 (Cat. No. AAA211182 Lot4) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-DHX9 antibody
DHX9 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAH box proteins also exhibit functions involved in transcriptional regulation. DHX9 has been shown to act as a bridge between CREB-binding protein (CBP) and RNA polymerase II to coactivate CBP-dependent transcription. A similar observation has been reported for an interaction with BRCA1. DHX9 also exhibits activities associated with nuclear export of retroviral RNAs, translation, RNA editing, and snRNP assembly.
Product Categories/Family for anti-DHX9 antibody
NCBI and Uniprot Product Information
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Product Notes
The DHX9 dhx9 (Catalog #AAA211182) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DHX9 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DHX9 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DHX9 dhx9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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