IHC (Immunohistochemisry)
(Anti- Dicer antibody, IHC(P)IHC(P): Human Mammary Cancer Tissue)
Dicer Polyclonal Antibody | anti-DICER1 antibody
Anti-Dicer Antibody
Gene Names
DICER1; DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Dicer, Antibody; Anti-Dicer Antibody; Endoribonuclease Dicer; DCR; DCR1; Dcr1 homolog (Drosophila); Dicer 1; Dicer 1 ribonuclease type III; Dicer; DICER_HUMAN; Dicer1; Double-strand-specific ribonuclease; Helicase moi; Helicase with RNAse motif; HERNA; KIAA0928; MNG1; RMSE2; dicer 1, ribonuclease type III; anti-DICER1 antibody
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
1829
Applicable Applications for anti-DICER1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti- Dicer antibody, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- Dicer antibody, IHC(P)IHC(P): Human Mammary Cancer Tissue)
WB (Western Blot)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlLane 1: Hela Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 217KDObserved bind size: 217KD)
WB (Western Blot)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlLane 1: Hela Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 217KDObserved bind size: 217KD)
WB (Western Blot)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlWB: Recombinant Human Dicer Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
WB (Western Blot)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlWB: Recombinant Human Dicer Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Related Product Information for anti-DICER1 antibody
Description: Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.
Background: Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Background: Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
References
1. Hutvagner, G., McLachlan, J., Pasquinelli, A. E., Balint, E., Tuschl, T., Zamore, P. D. A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA. Science 293: 834-838, 2001. 2. Matsuda, S., Ichigotani, Y., Okuda, T., Irimura, T., Nakatsugawa, S., Hamaguchi, M. Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase. Biochim. Biophys. Acta 1490: 163-169, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
endoribonuclease Dicer isoform 2
NCBI Official Synonym Full Names
dicer 1, ribonuclease III
NCBI Official Symbol
DICER1
NCBI Official Synonym Symbols
DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
NCBI Protein Information
endoribonuclease Dicer
UniProt Protein Name
Endoribonuclease Dicer
UniProt Gene Name
DICER1
UniProt Synonym Gene Names
DICER; HERNA; KIAA0928; Helicase MOI
UniProt Entry Name
DICER_HUMAN
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Product Notes
The DICER1 dicer1 (Catalog #AAA46418) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dicer can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DICER1 dicer1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dicer, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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