WB (Western Blot)
(Western blot analysis of Digoxigenin, pAb (Prod. No. AAA77056):Lane 1: MW markerLane 2: LTC4, mAb (Digoxingenin conjugate)Lane 3: IL-8, mAb (Digoxigenin conjugate).)
Sheep anti-General Digoxigenin Polyclonal Antibody
Digoxigenin polyclonal antibody
Reactivity
General
Applications
Western Blot, ELISA
Purity
Protein A affinity purified
Synonyms
Digoxigenin, Antibody; Digoxigenin polyclonal antibody; anti-Digoxigenin antibody
Host
Sheep
Reactivity
General
Clonality
Polyclonal
Specificity
Recognizes Digoxin and Digoxigenin
Purity/Purification
Protein A affinity purified
Form/Format
Liquid. In PBS containing 0.02% sodium azide
Applicable Applications for anti-Digoxigenin antibody
WB (Western Blot), ELISA
Source Note
Purified from sheep serum
Immunogen
Digoxin
Preparation and Storage
For long term storage, store at -20°C. For Short term Storage , Store at 4°C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of Digoxigenin, pAb (Prod. No. AAA77056):Lane 1: MW markerLane 2: LTC4, mAb (Digoxingenin conjugate)Lane 3: IL-8, mAb (Digoxigenin conjugate).)
WB (Western Blot)
(Western blot analysis of Digoxigenin, pAb (Prod. No. AAA77056):Lane 1: MW markerLane 2: LTC4, mAb (Digoxingenin conjugate)Lane 3: IL-8, mAb (Digoxigenin conjugate).)
IHC (Immunohistochemistry)
(Apoptotic nuclei in an ischemic model of brain injury visualized by TUNEL using digoxigenin polyclonal antibody (Prod. No. AAA77056) and horseradish peroxidase.)
IHC (Immunohistochemistry)
(Apoptotic nuclei in an ischemic model of brain injury visualized by TUNEL using digoxigenin polyclonal antibody (Prod. No. AAA77056) and horseradish peroxidase.)
Related Product Information for anti-Digoxigenin antibody
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. DIG is the steroid component of digoxin, a cardiac glycoside, and can be conjugated onto a variety of biomolecules (proteins, nucleic acids) to be detected in further assays (e.g. ELISA). These antibodies can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection.
DIG has an advantage over biotin due to the fact that biotin is an endogenous molecule of living cells and plays a key role in many reactions, which can sometimes lead to false positive results. Because the blossoms and leaves of Digitalis plants are the only natural sources of digoxigenin, binding of the anti-digoxigenin antibody in other biological material is not a consideration.
DIG has an advantage over biotin due to the fact that biotin is an endogenous molecule of living cells and plays a key role in many reactions, which can sometimes lead to false positive results. Because the blossoms and leaves of Digitalis plants are the only natural sources of digoxigenin, binding of the anti-digoxigenin antibody in other biological material is not a consideration.
Product Categories/Family for anti-Digoxigenin antibody
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Product Notes
The Digoxigenin (Catalog #AAA77056) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Digoxigenin polyclonal antibody reacts with General and may cross-react with other species as described in the data sheet. AAA Biotech's Digoxigenin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the Digoxigenin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Digoxigenin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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