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FCM/FACS (Flow Cytometry)
(DIO2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit DIO2 Polyclonal Antibody | anti-DIO2 antibody
DIO2 Antibody (Center)
Gene Names
DIO2; D2; 5DII; SelY; DIOII; TXDI2
Reactivity
Human (Predicted Reactivity: Pig)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
DIO2, Antibody; DIO2 Antibody (Center); Type II iodothyronine deiodinase; 5DII; DIOII; Type 2 DI; Type-II 5'-deiodinase; DIO2; ITDI2; TXDI2; anti-DIO2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Pig)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This DIO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-191 amino acids from the Central region of human DIO2.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
165-191
Sequence Length
309
Applicable Applications for anti-DIO2 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Function
Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
Cellular Location
Membrane; Single-pass membrane protein
Tissue Location
Isoform 1 is expressed in the lung, trachea, kidney, heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain (PubMed:11165050, PubMed:8755651). Isoform 2 is expressed in the brain, heart, kidney and trachea (PubMed:11165050)
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(DIO2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DIO2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(DIO2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(DIO2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of DIO2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DIO2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of DIO2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DIO2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(All lanes : Anti-DIO2 Antibody (Center) at 1:500-2000 dilutionLane 1: Mouse heart tissue lysateLane 2: Human skeletal muscle tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
WB (Western Blot)
(All lanes : Anti-DIO2 Antibody (Center) at 1:500-2000 dilutionLane 1: Mouse heart tissue lysateLane 2: Human skeletal muscle tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
WB (Western Blot)
(Western blot analysis of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody (Center). AAA285208 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody (Center). AAA285208 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-DIO2 antibody
DIO2 belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3).
Product Categories/Family for anti-DIO2 antibody
References
He,B.,et.al., Prog. Neuropsychopharmacol. Biol. Psychiatry 33 (6), 986-990 (2009)
Heemstra,K.A., et.al., J. Clin. Endocrinol. Metab. 94 (6), 2144-2150 (2009)
Heemstra,K.A., et.al., J. Clin. Endocrinol. Metab. 94 (6), 2144-2150 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
type II iodothyronine deiodinase isoform a
NCBI Official Synonym Full Names
deiodinase, iodothyronine, type II
NCBI Official Symbol
DIO2
NCBI Official Synonym Symbols
D2; 5DII; SelY; DIOII; TXDI2
NCBI Protein Information
type II iodothyronine deiodinase
UniProt Protein Name
Type II iodothyronine deiodinase
UniProt Gene Name
DIO2
UniProt Synonym Gene Names
ITDI2; TXDI2
UniProt Entry Name
IOD2_HUMAN
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Product Notes
The DIO2 dio2 (Catalog #AAA285208) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 165-191. The DIO2 Antibody (Center) reacts with Human (Predicted Reactivity: Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's DIO2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the DIO2 dio2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIO2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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