IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human DIO3 Polyclonal Antibody | anti-DIO3 antibody
DIO3 Antibody (C-term)
Gene Names
DIO3; D3; 5DIII; TXDI3; DIOIII
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DIO3, Antibody; DIO3 Antibody (C-term); Type III iodothyronine deiodinase; 5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase; DIO3; ITDI3; TXDI3; anti-DIO3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
250-278
Sequence Length
304
Applicable Applications for anti-DIO3 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of DIO3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). DIO3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of DIO3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). DIO3 (arrow) was detected using the purified Pab.)
Related Product Information for anti-DIO3 antibody
DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Product Categories/Family for anti-DIO3 antibody
References
Bessho, K., et al. Eur. J. Pediatr. 169(2):215-221(2010)
Wallace, C., et al. Nat. Genet. 42(1):68-71(2010)
Aerts, G., et al. Endocrinology 150(11):5171-5180(2009)
Panicker, V., et al. J. Clin. Endocrinol. Metab. 94(5):1623-1629(2009)
Panicker, V., et al. J. Clin. Endocrinol. Metab. 93(8):3075-3081(2008)
Wallace, C., et al. Nat. Genet. 42(1):68-71(2010)
Aerts, G., et al. Endocrinology 150(11):5171-5180(2009)
Panicker, V., et al. J. Clin. Endocrinol. Metab. 94(5):1623-1629(2009)
Panicker, V., et al. J. Clin. Endocrinol. Metab. 93(8):3075-3081(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33947
NCBI Official Full Name
type III iodothyronine deiodinase
NCBI Official Synonym Full Names
deiodinase, iodothyronine, type III
NCBI Official Symbol
DIO3
NCBI Official Synonym Symbols
D3; 5DIII; TXDI3; DIOIII
NCBI Protein Information
type III iodothyronine deiodinase
UniProt Protein Name
Type III iodothyronine deiodinase
UniProt Gene Name
DIO3
UniProt Synonym Gene Names
ITDI3; TXDI3
UniProt Entry Name
IOD3_HUMAN
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Product Notes
The DIO3 dio3 (Catalog #AAA286040) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 250-278. The DIO3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DIO3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DIO3 dio3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIO3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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