WB (Western Blot)
(Detection of human and mouse DIP2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DIP2B antibody AAA212830 (lot AAA212830-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse DIP2B Polyclonal Antibody | anti-DIP2B antibody
Rabbit anti-DIP2B Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DIP2B, Antibody; Rabbit anti-DIP2B Antibody, Affinity Purified; DIP2 homolog B; disco-interacting protein 2 homolog B; DIP2 disco-interacting protein 2 homolog B; Disco-interacting protein 2 homolog B; disco interacting protein 2 homolog B; anti-DIP2B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DIP2B antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 500 and 550
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse DIP2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DIP2B antibody AAA212830 (lot AAA212830-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse DIP2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DIP2B antibody AAA212830 (lot AAA212830-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DIP2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DIP2B antibody AAA212830 (lot AAA212830-1) used for IP at 6 ug per reaction. DIP2B was also immunoprecipitated by rabbit anti-DIP2B antibody BL17683. For blotting immunoprecipitated DIP2B, AAA212830 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DIP2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DIP2B antibody AAA212830 (lot AAA212830-1) used for IP at 6 ug per reaction. DIP2B was also immunoprecipitated by rabbit anti-DIP2B antibody BL17683. For blotting immunoprecipitated DIP2B, AAA212830 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-DIP2B antibody
Disco-interacting protein 2 homolog B (DIP2B) is a member of the disco-interacting protein homolog 2 protein family. DIP2B contains a binding site for the transcriptional regulator DNA methyltransferase 1 associated protein 1 as well as AMP-binding sites. The presence of these sites suggests thatDIP2B may participate in DNA methylation [taken from NCBI Entrez Gene (Gene ID: 57609)].
Product Categories/Family for anti-DIP2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
172 kDa
NCBI Official Full Name
disco-interacting protein 2 homolog B
NCBI Official Synonym Full Names
disco-interacting protein 2 homolog B
NCBI Official Symbol
DIP2B
NCBI Protein Information
disco-interacting protein 2 homolog B
UniProt Protein Name
Disco-interacting protein 2 homolog B
UniProt Gene Name
DIP2B
UniProt Synonym Gene Names
KIAA1463; DIP2 homolog B
UniProt Entry Name
DIP2B_HUMAN
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Product Notes
The DIP2B dip2b (Catalog #AAA212830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DIP2B Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DIP2B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DIP2B dip2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIP2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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