WB (Western Blot)
(Western blot analysis of extracts of mouse testis tissue, using DIS3 antibody.)
Rabbit DIS3 Polyclonal Antibody | anti-DIS3 antibody
DIS3 Antibody
Gene Names
DIS3; RRP44; dis3p; EXOSC11; KIAA1008; 2810028N01Rik
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DIS3, Antibody; DIS3 Antibody; 2810028N01Rik; bA555G22; Dis3; DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease; DIS3 mitotic control homolog (S. cerevisiae); DIS3 mitotic control homolog; DIS3 mitotic control, S. cerevisiae, homolog of; dis3p; EXOSC11; Exosome complex exonuclease RRP44; exosome component 11; KIAA1008; Mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA processing protein 44; Ribosomal RNA processing protein 44, S. cerevisiae, homolog of; Ribosomal RNA-processing protein 44; RP11-342J4.3; RRP44; RRP44_HUMAN; anti-DIS3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DIS3 antibody detects endogenous levels of DIS3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
928
Applicable Applications for anti-DIS3 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human DIS3.
Subcellular Location
Cytoplasm. Nucleus > Nucleolus. Nucleus > Nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3.
Tissue Specificity
Widely expressed.
Predicted Cross Reactivity
Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (91%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of mouse testis tissue, using DIS3 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of mouse testis tissue, using DIS3 antibody.)
IHC (Immunohistochemistry)
(AAA323207 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323207 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-DIS3 antibody
Function: Putative catalytic component of the RNA exosome complex which has 3'-> 5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
Subunit Structure: Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic-and nuclear-specific RNA exosome complex forms.
Similarity: Belongs to the RNR ribonuclease family.
Subunit Structure: Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic-and nuclear-specific RNA exosome complex forms.
Similarity: Belongs to the RNR ribonuclease family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 110 kDa
Predicted: 110 kDa
Predicted: 110 kDa
NCBI Official Full Name
exosome complex exonuclease RRP44 isoform b
NCBI Official Synonym Full Names
DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease
NCBI Official Symbol
DIS3
NCBI Official Synonym Symbols
RRP44; dis3p; EXOSC11; KIAA1008; 2810028N01Rik
NCBI Protein Information
exosome complex exonuclease RRP44
UniProt Protein Name
Exosome complex exonuclease RRP44
UniProt Gene Name
DIS3
UniProt Synonym Gene Names
KIAA1008; RRP44
Similar Products
Product Notes
The DIS3 dis3 (Catalog #AAA323207) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DIS3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DIS3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DIS3 dis3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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