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Knockout Validation
(Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: DLG5 knockout PC3 cell lysate; Predicted MW: 214kd Observed MW: 200kd Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Mouse Discs Polyclonal Antibody | anti-DLG5 antibody
Polyclonal Antibody to Discs, Large Homolog 5 (DLG5)
Gene Names
Dlg5; T25557; mKIAA0583; 4933429D20Rik
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Discs, Antibody; Polyclonal Antibody to Discs, Large Homolog 5 (DLG5); LP-DLG; P-DLG5; PDLG; Discs large protein P-dlg; Placenta and prostate DLG; anti-DLG5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against DLG5. It has been selected for its ability to recognize DLG5 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-DLG5 antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
DLG5 (Asp1724~Ser1917)
Conjugate
No Conjugate
Cross Reactivity
Human, Mouse
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant DLG5 (Asp1724~Ser1917) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: DLG5 knockout PC3 cell lysate; Predicted MW: 214kd Observed MW: 200kd Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: DLG5 knockout PC3 cell lysate; Predicted MW: 214kd Observed MW: 200kd Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-DLG5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
214,386 Da
NCBI Official Full Name
disks large homolog 5 isoform 1
NCBI Official Synonym Full Names
discs large MAGUK scaffold protein 5
NCBI Official Symbol
Dlg5
NCBI Official Synonym Symbols
T25557; mKIAA0583; 4933429D20Rik
NCBI Protein Information
disks large homolog 5
UniProt Protein Name
Disks large homolog 5
UniProt Gene Name
Dlg5
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Product Notes
The DLG5 dlg5 (Catalog #AAA145669) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Discs, Large Homolog 5 (DLG5) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Discs can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DLG5 dlg5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Discs, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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