IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DKC1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human, mouse DKC1 Polyclonal Antibody | anti-DKC1 antibody
DKC1 Antibody (Center)
Gene Names
DKC1; DKC; CBF5; DKCX; NAP57; NOLA4; XAP101
Reactivity
Human, mouse
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DKC1, Antibody; DKC1 Antibody (Center); H/ACA ribonucleoprotein complex subunit 4; 5499-; CBF5 homolog; Dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein NAP57; Nucleolar protein family A member 4; snoRNP protein DKC1; DKC1; NOLA4; anti-DKC1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DKC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 amino acids from the Central region of human DKC1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
185-213
Sequence Length
420
Applicable Applications for anti-DKC1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DKC1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DKC1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(DKC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DKC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(DKC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DKC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(DKC1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DKC1 antibody detected DKC1 protein (arrow).)
WB (Western Blot)
(DKC1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DKC1 antibody detected DKC1 protein (arrow).)
Related Product Information for anti-DKC1 antibody
DKC1 is a member of the H/ACA snoRNPs (small
nucleolar ribonucleoproteins) gene family. snoRNPs are involved in
various aspects of rRNA processing and modification and have been
classified into two families: C/D and H/ACA. The H/ACA snoRNPs also
include the NOLA1, 2 and 3 proteins. The protein encoded by this
gene and the three NOLA proteins localize to the dense fibrillar
components of nucleoli and to coiled (Cajal) bodies in the nucleus.
Both 18S rRNA production and rRNA pseudouridylation are impaired if
any one of the four proteins is depleted. These four H/ACA snoRNP
proteins are also components of the telomerase complex. The protein
encoded by this gene is related to the Saccharomyces cerevisiae
Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in
a tail-to-tail orientation with the palmitoylated erythrocyte
membrane protein gene and is transcribed in a telomere to
centromere direction. Both nucleotide substitutions and single
trinucleotide repeat polymorphisms have been found in this gene.
Mutations in this gene cause X-linked dyskeratosis congenita, a
disease resulting in reticulate skin pigmentation, mucosal
leukoplakia, nail dystrophy, and progressive bone marrow failure in
most cases.
nucleolar ribonucleoproteins) gene family. snoRNPs are involved in
various aspects of rRNA processing and modification and have been
classified into two families: C/D and H/ACA. The H/ACA snoRNPs also
include the NOLA1, 2 and 3 proteins. The protein encoded by this
gene and the three NOLA proteins localize to the dense fibrillar
components of nucleoli and to coiled (Cajal) bodies in the nucleus.
Both 18S rRNA production and rRNA pseudouridylation are impaired if
any one of the four proteins is depleted. These four H/ACA snoRNP
proteins are also components of the telomerase complex. The protein
encoded by this gene is related to the Saccharomyces cerevisiae
Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in
a tail-to-tail orientation with the palmitoylated erythrocyte
membrane protein gene and is transcribed in a telomere to
centromere direction. Both nucleotide substitutions and single
trinucleotide repeat polymorphisms have been found in this gene.
Mutations in this gene cause X-linked dyskeratosis congenita, a
disease resulting in reticulate skin pigmentation, mucosal
leukoplakia, nail dystrophy, and progressive bone marrow failure in
most cases.
References
Rostamiani, K., et al. Blood Cells Mol. Dis. 44 (2), 88 (2010) :
Grozdanov, P.N., et al. Hum. Mol. Genet. 18(23):4546-4551(2009)
Ashbridge, B., et al. Biochemistry 48(46):10858-10865(2009)
Sieron, P., et al. Br. J. Cancer 101(8):1410-1416(2009)
Grozdanov, P.N., et al. Hum. Mol. Genet. 18(23):4546-4551(2009)
Ashbridge, B., et al. Biochemistry 48(46):10858-10865(2009)
Sieron, P., et al. Br. J. Cancer 101(8):1410-1416(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57674
NCBI Official Full Name
H/ACA ribonucleoprotein complex subunit 4 isoform 1
NCBI Official Synonym Full Names
dyskeratosis congenita 1, dyskerin
NCBI Official Symbol
DKC1
NCBI Official Synonym Symbols
DKC; CBF5; DKCX; NAP57; NOLA4; XAP101
NCBI Protein Information
H/ACA ribonucleoprotein complex subunit 4
UniProt Protein Name
H/ACA ribonucleoprotein complex subunit 4
UniProt Gene Name
DKC1
UniProt Synonym Gene Names
NOLA4
UniProt Entry Name
DKC1_HUMAN
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Product Notes
The DKC1 dkc1 (Catalog #AAA285305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 185-213. The DKC1 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DKC1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DKC1 dkc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DKC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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