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WB (Western Blot)
(Western blot analysis of lysates from A549, Hela, HepG2, Jurkat, mouse NIH/3T3 cell line (from left to right), using DKK1 Antibody. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit anti-Human, mouse DKK1 Polyclonal Antibody | anti-DKK1 antibody
DKK1 Antibody
Gene Names
DKK1; SK; DKK-1
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DKK1, Antibody; DKK1 Antibody; Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK; DKK1; anti-DKK1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DKK1 antibody is generated from a rabbit immunized with a recombinant protein of human DKK1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
266
Applicable Applications for anti-DKK1 antibody
ELISA, WB (Western Blot)
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from A549, Hela, HepG2, Jurkat, mouse NIH/3T3 cell line (from left to right), using DKK1 Antibody. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A549, Hela, HepG2, Jurkat, mouse NIH/3T3 cell line (from left to right), using DKK1 Antibody. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Anti-DKK1 Antibody at 1:4000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-DKK1 Antibody at 1:4000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DKK1 Antibody at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DKK1 Antibody at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-DKK1 Antibody at 1:1000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-DKK1 Antibody at 1:1000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-DKK1 antibody
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Product Categories/Family for anti-DKK1 antibody
References
Fedi P.,et al.J. Biol. Chem. 274:19465-19472(1999).
Krupnik V.E.,et al.Gene 238:301-313(1999).
Tate G.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
Roessler E.,et al.Cytogenet. Cell Genet. 89:220-224(2000).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Krupnik V.E.,et al.Gene 238:301-313(1999).
Tate G.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
Roessler E.,et al.Cytogenet. Cell Genet. 89:220-224(2000).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28672
NCBI Official Full Name
dickkopf-related protein 1
NCBI Official Synonym Full Names
dickkopf WNT signaling pathway inhibitor 1
NCBI Official Symbol
DKK1
NCBI Official Synonym Symbols
SK; DKK-1
NCBI Protein Information
dickkopf-related protein 1
UniProt Protein Name
Dickkopf-related protein 1
UniProt Gene Name
DKK1
UniProt Synonym Gene Names
Dickkopf-1; Dkk-1; hDkk-1
UniProt Entry Name
DKK1_HUMAN
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Product Notes
The DKK1 dkk1 (Catalog #AAA285067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DKK1 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DKK1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DKK1 dkk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DKK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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