Rabbit anti-Human, Mouse DKK2 Polyclonal Antibody | anti-DKK2 antibody

DKK2 Antibody (N-term)

Cat. Num. AAA284333

• Gene Names: DKK2; DKK-2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Synonyms: DKK2, Antibody; DKK2 Antibody (N-term); Dickkopf-related protein 2; Dickkopf-2; Dkk-2; hDkk-2; DKK2; anti-DKK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This DKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-62 amino acids from the N-terminal region of human DKK2.
• Purity/Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 31-62
• Sequence Length: 259

• Applicable Applications for anti-DKK2 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity)
• Cellular Location: Secreted
• Tissue Location: Expressed in heart, brain, skeletal muscle and lung
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(DKK2 Antibody (T46) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the DKK2 antibody detected the DKK2 protein (arrow).)

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Western blot analysis of DKK2 (arrow) using DKK2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DKK2 gene (Lane 2) (Origene Technologies).)

WB (Western Blot)

(The anti-DKK2 Pab is used in Western blot to detect DKK2 in Jurkat cell lysate.)

Related Product Information for anti-DKK2 antibody

The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.

Product Categories/Family for anti-DKK2 antibody

Signal Transduction

References

Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Brott, B.K., et al., Mol. Cell. Biol. 22(17):6100-6110 (2002). Krupnik, V.E., et al., Gene 238(2):301-313 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 7657023
• NCBI GeneID: 27123
• NCBI Accession #: NP_055236.1
• NCBI GenBank Nucleotide #: NM_014421.2
• UniProt Accession #: Q9UBU2; Q9UIU3; A0AVE9; B2R6S7
• NCBI Official Full Name: dickkopf-related protein 2
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 2
• NCBI Official Symbol: DKK2
• NCBI Official Synonym Symbols: DKK-2
• NCBI Protein Information: dickkopf-related protein 2
• UniProt Protein Name: Dickkopf-related protein 2
• UniProt Gene Name: DKK2
• UniProt Synonym Gene Names: Dickkopf-2; Dkk-2; hDkk-2
• UniProt Entry Name: DKK2_HUMAN