IHC (Immunohiostchemistry)
(Anti- DLD Picoband antibody, AAA46498, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
DLD Polyclonal Antibody | anti-DLD antibody
Anti-DLD Antibody
Gene Names
DLD; E3; LAD; DLDD; DLDH; GCSL; PHE3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
DLD, Antibody; Anti-DLD Antibody; Dihydrolipoyl dehydrogenase; Diaphorase; Dihydrolipoamide dehydrogenase; Dihydrolipoyl dehydrogenase mitochondrial; dld; DLDH; DLDH_HUMAN; E3; E3 component of pyruvate dehydrogenase; E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex; GCSL; Glycine cleavage system L protein; Glycine cleavage system protein L; LAD; lipoamide dehydrogenase; Lipoamide reductase; Lipoyl dehydrogenase; mitochondrial; OTTHUMP00000206744; OTTHUMP00000206746; OTTHUMP00000206748; OTTHUMP00000206749; PHE 3; PHE3; dihydrolipoamide dehydrogenase; anti-DLD antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
509
Applicable Applications for anti-DLD antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human DLD recombinant protein (Position: K300-F509). Human DLD shares 96.2% and 95.7% amino acid (aa) sequence identity with mouse and rat DLD, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti- DLD Picoband antibody, AAA46498, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti- DLD Picoband antibody, AAA46498, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
WB (Western Blot)
(Anti- DLD Picoband antibody, AAA46498, Western blottingAll lanes: Anti DLD (AAA46498) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: Rat Testis Tissue Lysate at 50ugLane 4: Mouse Ovary Tissue Lysate at 50ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 54KDObserved bind size: 54KD)
WB (Western Blot)
(Anti- DLD Picoband antibody, AAA46498, Western blottingAll lanes: Anti DLD (AAA46498) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: Rat Testis Tissue Lysate at 50ugLane 4: Mouse Ovary Tissue Lysate at 50ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 54KDObserved bind size: 54KD)
Related Product Information for anti-DLD antibody
Description: Rabbit IgG polyclonal antibody for Dihydrolipoyl dehydrogenase, mitochondrial(DLD) detection. Tested with WB, IHC-P in Human;Mouse; Rat.
Background: DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
Background: DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
References
1. Feigenbaum, A. S., Robinson, B. H.The structure of the human dihydrolipoamide dehydrogenase gene (DLD) and its upstream elements.Genomics 17: 376-381, 1993. 2. Sakata, Y., Owada, Y., Sato, K., Kojima, K., Hisanaga, K., Shinka, T., Suzuki, Y., Aoki, Y., Satoh, J., Kondo, H., Matsubara, Y., Kure, S.Structure and expression of the glycine cleavage system in rat central nervous system.Molec. Brain Res. 94: 119-130, 2001. 3. Scherer, S. W., Otulakowski, G., Robinson, B. H., Tsui, L.-C.Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31-q32.Cytogenet. Cell Genet. 56: 176-177, 1991.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,283 Da
NCBI Official Full Name
dihydrolipoyl dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
dihydrolipoamide dehydrogenase
NCBI Official Symbol
DLD
NCBI Official Synonym Symbols
E3; LAD; DLDD; DLDH; GCSL; PHE3
NCBI Protein Information
dihydrolipoyl dehydrogenase, mitochondrial
UniProt Protein Name
Dihydrolipoyl dehydrogenase, mitochondrial
UniProt Gene Name
DLD
UniProt Synonym Gene Names
GCSL; LAD; PHE3
UniProt Entry Name
DLDH_HUMAN
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Product Notes
The DLD dld (Catalog #AAA46498) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-DLD Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DLD can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DLD dld for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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