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IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:50 (40x lens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) ( at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)
Rabbit DMD Polyclonal Antibody | anti-DMD antibody
DMD Polyclonal Antibody
Gene Names
DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Synonyms
DMD, Antibody; DMD Polyclonal Antibody; BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; anti-DMD antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Concentration
1mg/ml (varies by lot)
Sequence Length
3685
Applicable Applications for anti-DMD antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:1000
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
Species Reactivity/Cross Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 346-635 of human Dystrophin (NP_003997.2).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:50 (40x lens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) ( at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:50 (40x lens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) ( at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded Human skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:50 (40x lens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded Human skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:50 (40x lens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded rat heart using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded rat heart using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded mouse heart using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded mouse heart using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40xlens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40xlens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
WB (Western Blot)
(Western blot analysis of various lysates using Dystrophin Rabbit pAb (AAA10720) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of various lysates using Dystrophin Rabbit pAb (AAA10720) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Related Product Information for anti-DMD antibody
The dystrophin gene is the largest gene found in nature, measuring 2.4 Mb. The gene was identified through a positional cloning approach, targeted at the isolation of the gene responsible for Duchenne (DMD) and Becker (BMD) Muscular Dystrophies. DMD is a recessive, fatal, X-linked disorder occurring at a frequency of about 1 in 3,500 new-born males. BMD is a milder allelic form. In general, DMD patients carry mutations which cause premature translation termination (nonsense or frame shift mutations), while in BMD patients dystrophin is reduced either in molecular weight (derived from in-frame deletions) or in expression level. The dystrophin gene is highly complex, containing at least eight independent, tissue-specific promoters and two polyA-addition sites. Furthermore, dystrophin RNA is differentially spliced, producing a range of different transcripts, encoding a large set of protein isoforms. Dystrophin (as encoded by the Dp427 transcripts) is a large, rod-like cytoskeletal protein which is found at the inner surface of muscle fibers. Dystrophin is part of the dystrophin-glycoprotein complex (DGC), which bridges the inner cytoskeleton (F-actin) and the extra-cellular matrix. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-DMD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 427kDa
Calculated MW: 427kDa
Calculated MW: 427kDa
NCBI Official Full Name
Dystrophin
NCBI Official Synonym Full Names
dystrophin
NCBI Official Symbol
DMD
NCBI Official Synonym Symbols
BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
NCBI Protein Information
dystrophin
UniProt Protein Name
Dystrophin
UniProt Gene Name
DMD
UniProt Entry Name
DMD_HUMAN
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Product Notes
The DMD dmd (Catalog #AAA10720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DMD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200. Researchers should empirically determine the suitability of the DMD dmd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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