IHC (Immunohiostchemistry)
(AAA327738 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit DNAJA1 Polyclonal Antibody | anti-DNAJA1 antibody
DNAJA1 Antibody
Gene Names
DNAJA1; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSJ-2; HSPF4; NEDD7; hDJ-2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DNAJA1, Antibody; DNAJA1 Antibody; DJ 2; DJ2; DjA1; DnaJ (Hsp40) homolog, subfamily A, member 1; DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; DNAJ2; Dnaja1; DNJA1_HUMAN; hDJ 2; HDJ-2; HDJ2; Heat shock 40 kDa protein 4; heat shock protein DNAJ like 2; Heat shock protein J2; HSDJ; HSJ-2; HSJ2; HSPF4; Human DnaJ protein 2; NEDD7; Neural precursor cell expressed developmentally down regulated 7; OTTHUMP00000021193; anti-DNAJA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DNAJA1 antibody detects endogenous levels of total DNAJA1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
397
Applicable Applications for anti-DNAJA1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human DNAJA1
Subcellular Location
Membrane.
Tissue Specificity
Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA327738 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327738 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of DNAJA1 using 293 whole cell lysates)
WB (Western Blot)
(Western blot analysis of DNAJA1 using 293 whole cell lysates)
Related Product Information for anti-DNAJA1 antibody
Function: Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).
Subunit Structure: Identified in a complex with HSPA1B and BAX (PubMed:14752510). Interacts with RNF207 (PubMed:25281747).
Subunit Structure: Identified in a complex with HSPA1B and BAX (PubMed:14752510). Interacts with RNF207 (PubMed:25281747).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 44 kDa
Predicted: 45 kDa
Predicted: 45 kDa
NCBI Official Full Name
dnaJ homolog subfamily A member 1 isoform 1
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member A1
NCBI Official Symbol
DNAJA1
NCBI Official Synonym Symbols
DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSJ-2; HSPF4; NEDD7; hDJ-2
NCBI Protein Information
dnaJ homolog subfamily A member 1
UniProt Protein Name
DnaJ homolog subfamily A member 1
UniProt Gene Name
DNAJA1
UniProt Synonym Gene Names
DNAJ2; HDJ2; HSJ2; HSPF4; HSJ-2; hDj-2
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Product Notes
The DNAJA1 dnaja1 (Catalog #AAA327738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJA1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJA1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DNAJA1 dnaja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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