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product-image-AAA287776_FCM11.jpg FCM/FACS (Flow Cytometry) (DNAJB6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit anti-Human DNAJB6 Polyclonal Antibody | anti-DNAJB6 antibody

DNAJB6 Antibody (Center)

Gene Names
DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DNAJB6, Antibody; DNAJB6 Antibody (Center); DnaJ homolog subfamily B member 6; HHDJ1; Heat shock protein J2; HSJ-2; MRJ; MSJ-1; DNAJB6; HSJ2; MSJ1; anti-DNAJB6 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DNAJB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-247 amino acids from the Central region of human DNAJB6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
218-247
Sequence Length
277
Applicable Applications for anti-DNAJB6 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(DNAJB6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA287776_FCM11.jpg FCM/FACS (Flow Cytometry) (DNAJB6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(DNAJB6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNAJB6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

product-image-AAA287776_IHC13.jpg IHC (Immunohiostchemistry) (DNAJB6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNAJB6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of DNAJB6 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. DNAJB6 Antibody (Center) (arrow) was detected using the purified Pab.)

product-image-AAA287776_WB15.jpg WB (Western Blot) (Western blot analysis of DNAJB6 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. DNAJB6 Antibody (Center) (arrow) was detected using the purified Pab.)
Related Product Information for anti-DNAJB6 antibody
This gene encodes a member of the DNAJ protein family.
DNAJ family members are characterized by a highly conserved amino
acid stretch called the 'J-domain' and function as one of the two
major classes of molecular chaperones involved in a wide range of
cellular events, such as protein folding and oligomeric protein
complex assembly. This family member may also play a role in
polyglutamine aggregation in specific neurons. Alternative splicing
of this gene results in multiple transcript variants; however, not
all variants have been fully described.
Product Categories/Family for anti-DNAJB6 antibody
References
Mitra, A., et al. J. Biol. Chem. 285(32):24686-24694(2010)
Edo De Bock, C., et al. Int. J. Oncol. 36(5):1155-1163(2010)
Hageman, J., et al. Mol. Cell 37(3):355-369(2010)
Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009) :
Dey, S., et al. Mol. Cell. Biochem. 322 (1-2), 137-142 (2009) :

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36087
NCBI Official Full Name
dnaJ homolog subfamily B member 6 isoform b
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily B, member 6
NCBI Official Symbol
DNAJB6
NCBI Official Synonym Symbols
DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E
NCBI Protein Information
dnaJ homolog subfamily B member 6
UniProt Protein Name
DnaJ homolog subfamily B member 6
UniProt Gene Name
DNAJB6
UniProt Synonym Gene Names
HSJ2; MRJ; MSJ1; HSJ-2
UniProt Entry Name
DNJB6_HUMAN

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Product Notes

The DNAJB6 dnajb6 (Catalog #AAA287776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 218-247. The DNAJB6 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJB6 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DNAJB6 dnajb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJB6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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