IHC (Immunohiostchemistry)
(AAA323093 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit DNAJC10 Polyclonal Antibody | anti-DNAJC10 antibody
DNAJC10 Antibody
Gene Names
DNAJC10; JPDI; MTHr; ERdj5; PDIA19
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DNAJC10, Antibody; DNAJC10 Antibody; DKFZp434J1813; DnaJ (Hsp40) homolog subfamily C member 10; DnaJ homolog subfamily C member 10; DNAJC 10; ER resident protein ERdj5; ERdj 5; ERdj5; J domain containing protein disulfide isomerase like protein; JPDI; Macrothioredoxin; MGC104194; MTHr; anti-DNAJC10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DNAJC10 antibody detects endogenous levels of DNAJC10.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
747
Applicable Applications for anti-DNAJC10 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human DNAJC10.
Subcellular Location
Secreted. Endoplasmic Reticulum Lumen.
Tissue Specificity
By endoplasmic reticulum stress.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (90%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (91%), Xenopus (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA323093 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323093 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts of mouse liver tissue, using DNAJC10 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of mouse liver tissue, using DNAJC10 antibody.)
Related Product Information for anti-DNAJC10 antibody
Function: Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.
Subunit Structure: Interacts with EDEM1 (By similarity). Interacts with HSPA5 (via its J domain).
Similarity: The thioredoxin-like regions Trxb 1 and 2 lack a redox-active CXXC motif.Thioredoxin domains 3 and 4 are the primary reductase domains.
Subunit Structure: Interacts with EDEM1 (By similarity). Interacts with HSPA5 (via its J domain).
Similarity: The thioredoxin-like regions Trxb 1 and 2 lack a redox-active CXXC motif.Thioredoxin domains 3 and 4 are the primary reductase domains.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 75-80 kDa
Predicted: 92 kDa
Predicted: 92 kDa
NCBI Official Full Name
dnaJ homolog subfamily C member 10 isoform 2
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member C10
NCBI Official Symbol
DNAJC10
NCBI Official Synonym Symbols
JPDI; MTHr; ERdj5; PDIA19
NCBI Protein Information
dnaJ homolog subfamily C member 10
UniProt Protein Name
DnaJ homolog subfamily C member 10
UniProt Gene Name
DNAJC10
UniProt Synonym Gene Names
ERDJ5; ER-resident protein ERdj5; ERdj5; MTHr
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Product Notes
The DNAJC10 dnajc10 (Catalog #AAA323093) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJC10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJC10 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DNAJC10 dnajc10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJC10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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