WB (Western Blot)
(Detection of human DNAM-1/CD226 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-DNAM-1/CD226 antibody AAA212644 (lot AAA212644-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human DNAM-1/CD226 Polyclonal Antibody | anti-DNAM-1/CD226 antibody
Rabbit anti-DNAM-1/CD226 Antibody, Affinity Purified
Gene Names
CD226; PTA1; DNAM1; DNAM-1; TLiSA1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DNAM-1/CD226, Antibody; Rabbit anti-DNAM-1/CD226 Antibody, Affinity Purified; DNAM-1; platelet and T cell activation antigen 1; TLiSA1; T lineage-specific activation antigen 1 antigen; PTA1; adhesion glycoprotein; DNAM1; CD226 antigen; CD antigen CD226; DNAX accessory molecule 1; DNAX accessory molecule-1; CD226 molecule; anti-DNAM-1/CD226 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DNAM-1/CD226 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Monkey
Immunogen
Between 286 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DNAM-1/CD226 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-DNAM-1/CD226 antibody AAA212644 (lot AAA212644-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human DNAM-1/CD226 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-DNAM-1/CD226 antibody AAA212644 (lot AAA212644-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human DNAM-1/CD226 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-DNAM-1/CD226 antibody AAA212644 (lot AAA212644-1) used for IP at 6 ug per reaction. DNAM-1/CD226 was also immunoprecipitated by rabbit anti-DNAM-1/CD226 antibody BL16224. For blotting immunoprecipitated DNAM-1/CD226, AAA212644 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DNAM-1/CD226 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-DNAM-1/CD226 antibody AAA212644 (lot AAA212644-1) used for IP at 6 ug per reaction. DNAM-1/CD226 was also immunoprecipitated by rabbit anti-DNAM-1/CD226 antibody BL16224. For blotting immunoprecipitated DNAM-1/CD226, AAA212644 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-DNAM-1/CD226 antibody
DNAX accessory molecule-1 (DNAM-1) is a receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15762].
Product Categories/Family for anti-DNAM-1/CD226 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,614 Da
NCBI Official Full Name
CD226 antigen
NCBI Official Synonym Full Names
CD226 molecule
NCBI Official Symbol
CD226
NCBI Official Synonym Symbols
PTA1; DNAM1; DNAM-1; TLiSA1
NCBI Protein Information
CD226 antigen; adhesion glycoprotein; DNAX accessory molecule 1; DNAX accessory molecule-1; platelet and T cell activation antigen 1; T lineage-specific activation antigen 1 antigen
UniProt Protein Name
CD226 antigen
UniProt Gene Name
CD226
UniProt Synonym Gene Names
DNAM1; DNAM-1
UniProt Entry Name
CD226_HUMAN
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Product Notes
The DNAM-1/CD226 cd226 (Catalog #AAA212644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DNAM-1/CD226 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAM-1/CD226 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DNAM-1/CD226 cd226 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAM-1/CD226, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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