WB (Western Blot)
(Detection of human DNL3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNL3 antibody (AAA211563 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human DNL3 Polyclonal Antibody | anti-DNL3 antibody
Rabbit anti-DNL3 Antibody, Affinity Purified
Gene Names
LIG3; LIG2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DNL3, Antibody; Rabbit anti-DNL3 Antibody, Affinity Purified; DNA ligase III; ligase III, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 3; ligase II, DNA, ATP-dependent; DNA ligase 3; LIG2; LIG3alpha; anti-DNL3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DNL3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 900 and 950
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DNL3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNL3 antibody (AAA211563 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human DNL3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNL3 antibody (AAA211563 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human DNL3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNL3 antibody (AAA211563 lot 3) used for IP at 6 ug per reaction. DNL3 was also immunoprecipitated by a previous lot of this antibody (AAA211563 lot 2) and a second antibody against a different epitope of DNL3 (BL6291). For blotting immunoprecipitated DNL3, AAA211563 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DNL3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNL3 antibody (AAA211563 lot 3) used for IP at 6 ug per reaction. DNL3 was also immunoprecipitated by a previous lot of this antibody (AAA211563 lot 2) and a second antibody against a different epitope of DNL3 (BL6291). For blotting immunoprecipitated DNL3, AAA211563 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-DNL3 antibody
DNA ligase III catalyzes the joining of DNA ends during DNA strand-break repair. DNA ligase III interacts with the DNA-repair factor XRCC1 to function in base excision repair (BER). DNA ligase III has also been shown to play a required role in microhomology-mediated end-joining of double-strand breaks.
Product Categories/Family for anti-DNL3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
112,907 Da
NCBI Official Full Name
DNA ligase III
NCBI Official Synonym Full Names
ligase III, DNA, ATP-dependent
NCBI Official Symbol
LIG3
NCBI Official Synonym Symbols
LIG2
NCBI Protein Information
DNA ligase 3; OTTHUMP00000158641; OTTHUMP00000163854; ligase II, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 3
UniProt Protein Name
DNA ligase 3
UniProt Gene Name
LIG3
UniProt Entry Name
DNLI3_HUMAN
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Product Notes
The DNL3 lig3 (Catalog #AAA211563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DNL3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNL3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DNL3 lig3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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