IHC (Immunohistochemisry)
(DOC2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOC2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit DOC2A Polyclonal Antibody | anti-DOC2A antibody
DOC2A Antibody (Center)
Gene Names
DOC2A; Doc2
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DOC2A, Antibody; DOC2A Antibody (Center); Double C2-like domain-containing protein alpha; Doc2; Doc2-alpha; DOC2A; anti-DOC2A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DOC2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human DOC2A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
277-306
Sequence Length
400
Applicable Applications for anti-DOC2A antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(DOC2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOC2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(DOC2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOC2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(DOC2A Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DOC2A antibody detected the DOC2A protein (arrow).)
WB (Western Blot)
(DOC2A Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DOC2A antibody detected the DOC2A protein (arrow).)
FCM/FACS (Flow Cytometry)
(DOC2A Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DOC2A Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-DOC2A antibody
There are at least two protein isoforms of the Double C2
protein, namely alpha (DOC2A) and beta (DOC2B), which contain two
C2-like domains. DOC2A and DOC2B are encoded by different genes;
these genes are at times confused with the unrelated DAB2 gene
which was initially named DOC-2. DOC2A is mainly expressed in brain
and is suggested to be involved in Ca(2+)-dependent
neurotransmitter release.
protein, namely alpha (DOC2A) and beta (DOC2B), which contain two
C2-like domains. DOC2A and DOC2B are encoded by different genes;
these genes are at times confused with the unrelated DAB2 gene
which was initially named DOC-2. DOC2A is mainly expressed in brain
and is suggested to be involved in Ca(2+)-dependent
neurotransmitter release.
References
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Glessner, J.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(23):10584-10589(2010)
Guilmatre, A., et al. Arch. Gen. Psychiatry 66(9):947-956(2009)
Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009) :
Higashio, H., et al. J. Immunol. 180(7):4774-4784(2008)
Glessner, J.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(23):10584-10589(2010)
Guilmatre, A., et al. Arch. Gen. Psychiatry 66(9):947-956(2009)
Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009) :
Higashio, H., et al. J. Immunol. 180(7):4774-4784(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43959
NCBI Official Full Name
double C2-like domain-containing protein alpha
NCBI Official Synonym Full Names
double C2-like domains, alpha
NCBI Official Symbol
DOC2A
NCBI Official Synonym Symbols
Doc2
NCBI Protein Information
double C2-like domain-containing protein alpha
UniProt Protein Name
Double C2-like domain-containing protein alpha
UniProt Gene Name
DOC2A
UniProt Synonym Gene Names
Doc2; Doc2-alpha
UniProt Entry Name
DOC2A_HUMAN
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Product Notes
The DOC2A doc2a (Catalog #AAA288612) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 277-306. The DOC2A Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's DOC2A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the DOC2A doc2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DOC2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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