WB (Western Blot)
(Detection of human DOCK10 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK10 antibody AAA211409 (lot AAA211409-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human DOCK10 Polyclonal Antibody | anti-DOCK10 antibody
Rabbit anti-DOCK10 Antibody, Affinity Purified
Gene Names
DOCK10; ZIZ3; DRIP2; Nbla10300; DKFZp781A1532
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DOCK10, Antibody; Rabbit anti-DOCK10 Antibody, Affinity Purified; dopamine receptor interacting protein 2; DRIP2; Nbla10300; ZIZ3; zizimin3; Zizimin-3; dedicator of cytokinesis protein 10; Dedicator of cytokinesis protein 10; dedicator of cytokinesis 10; anti-DOCK10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DOCK10 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DOCK10 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK10 antibody AAA211409 (lot AAA211409-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human DOCK10 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK10 antibody AAA211409 (lot AAA211409-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human DOCK10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK10 antibody AAA211409 (lot AAA211409-2) used for IP at 6 ug per reaction. DOCK10 was also immunoprecipitated by a previous lot of this antibody (lot AAA211409-1). For blotting immunoprecipitated DOCK10, AAA211409 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DOCK10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK10 antibody AAA211409 (lot AAA211409-2) used for IP at 6 ug per reaction. DOCK10 was also immunoprecipitated by a previous lot of this antibody (lot AAA211409-1). For blotting immunoprecipitated DOCK10, AAA211409 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-DOCK10 antibody
DOCK10 is a potential Rho-guanine nucleotide exchange factor (Rho-GEF) that may function to activate small GTPases such as Rac and Cdc42. It is a member of the CZH (CDM-zizimin homology) family of proteins that contain two highly conserved domains, CZH1 and CZH2.
Product Categories/Family for anti-DOCK10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
249,531 Da
NCBI Official Full Name
dedicator of cytokinesis protein 10
NCBI Official Synonym Full Names
dedicator of cytokinesis 10
NCBI Official Symbol
DOCK10
NCBI Official Synonym Symbols
ZIZ3; DRIP2; Nbla10300; DKFZp781A1532
NCBI Protein Information
dedicator of cytokinesis protein 10; zizimin3; zizimin-3; protein zizimin 3; OTTHUMP00000203874; OTTHUMP00000203922; dopamine receptor interacting protein 2
UniProt Protein Name
Dedicator of cytokinesis protein 10
UniProt Gene Name
DOCK10
UniProt Synonym Gene Names
KIAA0694; ZIZ3
UniProt Entry Name
DOC10_HUMAN
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Product Notes
The DOCK10 dock10 (Catalog #AAA211409) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DOCK10 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DOCK10 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DOCK10 dock10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DOCK10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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