Rabbit anti-Rat Dopamine Transporter (Thr53) Polyclonal Antibody | anti-SLC6A3 antibody

Anti-Phospho-Thr53 Dopamine Transporter (DAT)

Cat. Num. AAA72821

• Gene Names: SLC6A3; DAT; DAT1; PKDYS
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Dopamine Transporter (Thr53), Antibody; Anti-Phospho-Thr53 Dopamine Transporter (DAT); anti-SLC6A3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. Immunolabeling of the DAT band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 620

• Applicable Applications for anti-SLC6A3 antibody: WB (Western Blot)
• Preparation and Storage: For long term storage-80 degree C is recommended, but shorter term storage at -20 degree C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 degree C.

Application Data

Related Product Information for anti-SLC6A3 antibody

Affinity purified rabbit polyclonal antibody

References

• Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
• Wilson JM, Kalasinsky KS, Levey AI, Bergeron C, Reiber G, Anthony RM, Schmunk GA, Shannak K, Haycock JW, Kish SJ (1996) Striatal dopamine nerve terminal markers in human, chronic methamphetamine users. Nat Med 2:699-703.
• Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
• Zhu MY, Klimek V, Dilley GE, Haycock JW, StockmeierC, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.
• James D. Foster, Jae-Won Yang, Amy E. Moritz, Sathyavathi ChallaSivaKanaka, Margaret A. Smith, Marion Holy, Kyle Wilebski, Harald H. Sitte, and Roxanne A. Vaughan (2012) Dopamine Transporter Phosphorylation Site Threonine 53 Regulates Substrate Reuptake and Amphetamine-stimulated Efflux. Journal of Biological Chemistry 287(35):29702-29712.

NCBI and Uniprot Product Information

• NCBI GI #: 7108463
• NCBI GeneID: 6531
• UniProt Accession #: Q01959; Q14996; A2RUN4
• NCBI Official Full Name: dopamine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 3
• NCBI Official Symbol: SLC6A3
• NCBI Official Synonym Symbols: DAT; DAT1; PKDYS
• NCBI Protein Information: sodium-dependent dopamine transporter; DA transporter; dopamine transporter 1; solute carrier family 6 member 3; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• UniProt Protein Name: Sodium-dependent dopamine transporter
• UniProt Gene Name: SLC6A3
• UniProt Synonym Gene Names: DAT1; DA transporter; DAT
• UniProt Entry Name: SC6A3_HUMAN