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IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
Doublecortin/DCX Polyclonal Antibody | anti-DCX antibody
Goat anti-Doublecortin/DCX (aa232-242) Antibody
Gene Names
DCX; DC; DBCN; LISX; SCLH; XLIS
Reactivity
Species Reactivity: Mouse, Human Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Doublecortin/DCX, Antibody; Goat anti-Doublecortin/DCX (aa232-242) Antibody; DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX,SCLH, XLIS, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X,neuronal migration protein doublecortin; anti-DCX antibody
Reactivity
Species Reactivity: Mouse, Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Applicable Applications for anti-DCX antibody
Pep-ELISA, Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Mouse NP_001103692.1.
Recommended concentration: 0.01-0.03ug/ml.
Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration, 2-4ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5ug/ml
Western blot: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Mouse NP_001103692.1.
Recommended concentration: 0.01-0.03ug/ml.
Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration, 2-4ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5ug/ml
Immunogen
Peptide with sequence C-KTSANMKAPQS, from the internal region of the protein sequence according to NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1.
Note
This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
IHC (Immunohistochemistry)
(AAA13668 (2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
(AAA13668 (2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
WB (Western Blot)
((0. 01ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0. 01ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15%Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15%Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
neuronal migration protein doublecortin isoform a
NCBI Official Synonym Full Names
doublecortin
NCBI Official Symbol
DCX
NCBI Official Synonym Symbols
DC; DBCN; LISX; SCLH; XLIS
NCBI Protein Information
neuronal migration protein doublecortin
UniProt Protein Name
Neuronal migration protein doublecortin
UniProt Gene Name
DCX
UniProt Synonym Gene Names
DBCN; LISX; Lis-X
UniProt Entry Name
DCX_HUMAN
Similar Products
Product Notes
The DCX dcx (Catalog #AAA13668) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Doublecortin/DCX (aa232-242) Antibody reacts with Species Reactivity: Mouse, Human Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Doublecortin/DCX can be used in a range of immunoassay formats including, but not limited to, Pep-ELISA, Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Mouse NP_001103692.1. Recommended concentration: 0.01-0.03ug/ml.Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration, 2-4ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5ug/ml. Researchers should empirically determine the suitability of the DCX dcx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Doublecortin/DCX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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