Rabbit DSG4/Desmoglein 4 Polyclonal Antibody | anti-DSG4 antibody

Anti-DSG4/Desmoglein 4 Antibody (Internal)

Cat. Num. AAA162393

• Gene Names: DSG4; LAH; HYPT6; CDGF13; CDHF13
• Reactivity: Human, Mouse, Rat.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: DSG4/Desmoglein 4, Antibody; Anti-DSG4/Desmoglein 4 Antibody (Internal); Rabbit Polyclonal to Human DSG4/Desmoglein 4; DSG4; Cadherin family member 13; CDGF13; CDH family member 13; Desmoglein 4; Desmoglein-4; LAH; CDHF13; anti-DSG4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat.
• Clonality: Polyclonal
• Specificity: Recognizes endogeneous levels of Desmoglein 4 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
• Sequence Length: 1059

• Applicable Applications for anti-DSG4 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen Species: DSG4/ Desmoglein 4 antibody was raised against human.
• Immunogen: DSG4/ Desmoglein 4 antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the central region of human Desmoglein 4.
• Target: DSG4/Desmoglein 4
• Epitope: Internal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C.; Aliquot to avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of Desmoglein 4 expression in HEK293T (A); SP2/0 (B); H9C2 (C) whole cell lysates.)

IHC (Immunohiostchemistry)

(Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

NCBI and Uniprot Product Information

• NCBI GI #: 197313787
• NCBI GeneID: 147409
• NCBI Accession #: NP_001127925.1
• NCBI GenBank Nucleotide #: NM_001134453.2
• UniProt Accession #: Q86SJ6; Q6Y9L9; Q8IXV4; A2RUI1
• NCBI Official Full Name: desmoglein-4 isoform 1
• NCBI Official Synonym Full Names: desmoglein 4
• NCBI Official Symbol: DSG4
• NCBI Official Synonym Symbols: LAH; HYPT6; CDGF13; CDHF13
• NCBI Protein Information: desmoglein-4
• UniProt Protein Name: Desmoglein-4
• UniProt Gene Name: DSG4
• UniProt Synonym Gene Names: CDHF13