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WB (Western Blot)
(Detection of human DTL/CDT2 by western blot. Samples: Whole cell lysate (50 ug) from U2OS, HeLa, Jurkat, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL-CDT2 antibody (AAA211240 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human DTL/CDT2 Polyclonal Antibody | anti-DTL/CDT2 antibody
Rabbit anti-DTL/CDT2 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DTL/CDT2, Antibody; Rabbit anti-DTL/CDT2 Antibody, Affinity Purified; RAMP; RA-regulated nuclear matrix-associated protein; lethal(2) denticleless protein homolog; L2DTL; DDB1- and CUL4-associated factor 2; DCAF2; CDT2; denticleless protein homolog; retinoic acid-regulated nuclear matrix-associated protein; Denticleless protein homolog; denticleless E3 ubiquitin protein ligase homolog; anti-DTL/CDT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DTL/CDT2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 680 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DTL/CDT2 by western blot. Samples: Whole cell lysate (50 ug) from U2OS, HeLa, Jurkat, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL-CDT2 antibody (AAA211240 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human DTL/CDT2 by western blot. Samples: Whole cell lysate (50 ug) from U2OS, HeLa, Jurkat, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL-CDT2 antibody (AAA211240 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody (AAA211240 lot 4) used for IP at 6 ug per reaction. DTL/CDT2 was also immunoprecipitated by a previous lot of this antibody (AAA211240 lot 3). For blotting immunoprecipitated DTL/CDT2, AAA211240 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody (AAA211240 lot 4) used for IP at 6 ug per reaction. DTL/CDT2 was also immunoprecipitated by a previous lot of this antibody (AAA211240 lot 3). For blotting immunoprecipitated DTL/CDT2, AAA211240 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohiostchemistry)
(Detection of mouse DTL/CDT2 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit antiDTL/CDT2 (AAA211240 lot 4) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse DTL/CDT2 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit antiDTL/CDT2 (AAA211240 lot 4) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DTL/CDT2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit antiDTL/CDT2 (AAA211240 lot 4) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DTL/CDT2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit antiDTL/CDT2 (AAA211240 lot 4) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
Related Product Information for anti-DTL/CDT2 antibody
DTL/CDT2 is a component of the CUL4A-DDB1 E3 ubiquitinating complex and plays a role in downregulating CDT1, an essential component of pre-replication complexes (Pre-RC) that ensures the genome is replicated only once per cycle. As part of the CUL4A-DDB1 E3 ligase complex DTL/CDT2 is also involved in the regulation of other proteins involved in the G2/M cell cycle checkpoint.
Product Categories/Family for anti-DTL/CDT2 antibody
NCBI and Uniprot Product Information
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Product Notes
The DTL/CDT2 dtl (Catalog #AAA211240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DTL/CDT2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DTL/CDT2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DTL/CDT2 dtl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DTL/CDT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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