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IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells using AAA230285 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human Dual specificity mitogen-activated protein kinase kinase 3 Polyclonal Antibody | anti-MAP2K3 antibody
Rabbit anti-human Dual specificity mitogen-activated protein kinase kinase 3 polyclonal Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Dual specificity mitogen-activated protein kinase kinase 3, Antibody; Rabbit anti-human Dual specificity mitogen-activated protein kinase kinase 3 polyclonal Antibody; MAPK/ERK kinase 3 MAP2K3 MEK3; MKK3; PRKMK3; SKK2; anti-MAP2K3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
318
Applicable Applications for anti-MAP2K3 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Dual specificity mitogen-activated protein kinase kinase 3 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-13069 / sc-961 / sc-959 / sc-960
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells using AAA230285 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells using AAA230285 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA230285 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA230285 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded AAA230285 using human skeletal muscle tissue at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded AAA230285 using human skeletal muscle tissue at dilution 1:100)
WB (Western Blot)
(Western blotAll lanes: MAP2K3 antibody at 10 ug/mlLane 1: Raw264.7 whole cell lysateLane 2: Mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 40,37 kDaObserved band size: 38,26 kDa)
WB (Western Blot)
(Western blotAll lanes: MAP2K3 antibody at 10 ug/mlLane 1: Raw264.7 whole cell lysateLane 2: Mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 40,37 kDaObserved band size: 38,26 kDa)
Related Product Information for anti-MAP2K3 antibody
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
This protein is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
This protein is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Product Categories/Family for anti-MAP2K3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,940 Da
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 3 isoform A
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 3
NCBI Official Symbol
MAP2K3
NCBI Official Synonym Symbols
MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 3
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 3
UniProt Gene Name
MAP2K3
UniProt Synonym Gene Names
MEK3; MKK3; PRKMK3; SKK2; MAP kinase kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; SAPKK-2; SAPKK2
UniProt Entry Name
MP2K3_HUMAN
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Product Notes
The MAP2K3 map2k3 (Catalog #AAA230285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Dual specificity mitogen-activated protein kinase kinase 3 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dual specificity mitogen-activated protein kinase kinase 3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the MAP2K3 map2k3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dual specificity mitogen-activated protein kinase kinase 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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