IP (Immunoprecipitation)
(VHR was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate1 uL anti-VHR rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-VHR rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Rabbit anti-Human DUSP3 Polyclonal Antibody | anti-DUSP3 antibody
Anti-DUSP3 Antibody, Rabbit Polyclonal
Gene Names
DUSP3; VHR
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
DUSP3, Antibody; Anti-DUSP3 Antibody, Rabbit Polyclonal; DUSP3/VHR Antibody, Rabbit PAb, Antigen Affinity Purified; dual specificity phosphatase 3; Anti-VHR Antibody; anti-DUSP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DUSP3/VHR
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-DUSP3 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human DUSP3/VHR Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human DUSP3/VHR (rh DUSP3/VHR). DUSP3/VHR specific IgG was purified by Human DUSP3/VHR affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(VHR was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate1 uL anti-VHR rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-VHR rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
IP (Immunoprecipitation)
(VHR was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate1 uL anti-VHR rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-VHR rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
WB (Western Blot)
(Anti-VHR rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:20 kDaObserved band size:22 kDa)
WB (Western Blot)
(Anti-VHR rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:20 kDaObserved band size:22 kDa)
Related Product Information for anti-DUSP3 antibody
Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP), and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs, VHR lacks mitogen-activated protein kinase (MAPK)-binding domain, and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates, it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs). VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and, therefore, may play a role in aspects of T lymphocyte physiology that depend on these kinases.
Product Categories/Family for anti-DUSP3 antibody
References
Todd J.L, et al. (1999) Extracellular regulated kinases (ERK) 1 and ERK2 are authentic substrates for the dual-specificity protein-tyrosine phosphatase VHR. A novel role in down-regulating the ERK pathway. J. Biol. Chem. 274: 13271-80.Alonso A, et al. (2001) Inhibitory role for dual specificity phosphatase VHR in T cell antigen receptor and CD28-induced Erk and Jnk activation. J Biol Chem. 276(7): 4766-71.Schumacher MA, et al. (2002) Structural basis for the recognition of a bisphosphorylated MAP kinase peptide by human VHR protein Phosphatase. Biochemistry. 41(9): 3009-17.Patterson KI, et al. (2009) Dual-specificity phosphatases: critical regulators with diverse cellular targets. Biochem J. 2009 Mar 15;418(3): 475-89.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,163 Da
NCBI Official Full Name
dual specificity protein phosphatase 3
NCBI Official Synonym Full Names
dual specificity phosphatase 3
NCBI Official Symbol
DUSP3
NCBI Official Synonym Symbols
VHR
NCBI Protein Information
dual specificity protein phosphatase 3; dual specificity protein phosphatase VHR; serine/threonine specific protein phosphatase; vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related
UniProt Protein Name
Dual specificity protein phosphatase 3
UniProt Gene Name
DUSP3
UniProt Synonym Gene Names
VHR; VHR
UniProt Entry Name
DUS3_HUMAN
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Product Notes
The DUSP3 dusp3 (Catalog #AAA254480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DUSP3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DUSP3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DUSP3 dusp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DUSP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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