IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DVL3 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Rabbit DVL3 Polyclonal Antibody | anti-DVL3 antibody
DVL3 Antibody (C-term)
Reactivity
Human, mouse (Predicted Reactivity: Mouse)
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DVL3, Antibody; DVL3 Antibody (C-term); Segment polarity protein dishevelled homolog DVL-3; Dishevelled-3; DSH homolog 3; DVL3; KIAA0208; anti-DVL3 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DVL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-557 amino acids from the C-terminal region of human DVL3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
530-557
Sequence Length
699
Applicable Applications for anti-DVL3 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DVL3 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DVL3 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(DVL3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DVL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(DVL3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DVL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(All lanes : Anti-DVL3 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLane 3: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 78 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DVL3 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLane 3: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 78 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-DVL3 antibody
This gene is a member of a multi-gene family which shares
strong similarity with the Drosophila dishevelled gene, dsh. The
Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein
that regulates cell proliferation.
strong similarity with the Drosophila dishevelled gene, dsh. The
Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein
that regulates cell proliferation.
Product Categories/Family for anti-DVL3 antibody
References
Gnad, T., et al. Mol. Cancer 9, 31 (2010) :
Bouteille, N., et al. Oncogene 28(28):2569-2580(2009)
Fukukawa, C., et al. Oncogene 28(8):1110-1120(2009)
Wei, Q., et al. Lung Cancer 62(2):181-192(2008)
Lee, Y.N., et al. Cell. Signal. 20(2):443-452(2008)
Bouteille, N., et al. Oncogene 28(28):2569-2580(2009)
Fukukawa, C., et al. Oncogene 28(8):1110-1120(2009)
Wei, Q., et al. Lung Cancer 62(2):181-192(2008)
Lee, Y.N., et al. Cell. Signal. 20(2):443-452(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78055
NCBI Official Full Name
segment polarity protein dishevelled homolog DVL-3
NCBI Official Synonym Full Names
dishevelled segment polarity protein 3
NCBI Official Symbol
DVL3
NCBI Protein Information
segment polarity protein dishevelled homolog DVL-3
UniProt Protein Name
Segment polarity protein dishevelled homolog DVL-3
UniProt Gene Name
DVL3
UniProt Synonym Gene Names
KIAA0208; Dishevelled-3
UniProt Entry Name
DVL3_HUMAN
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Product Notes
The DVL3 dvl3 (Catalog #AAA288386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 530-557. The DVL3 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's DVL3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DVL3 dvl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DVL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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