IHC (Immunohistochemisry)
(AAA323526 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse DYRK4 Polyclonal Antibody | anti-DYRK4 antibody
DYRK4 Antibody
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DYRK4, Antibody; DYRK4 Antibody; Dual specificity tyrosine phosphorylation regulated kinase 4; Dual specificity tyrosine-phosphorylation-regulated kinase 4; Dyrk4; DYRK4_HUMAN; EC 2.7.12.1; anti-DYRK4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
DYRK4 antibody detects endogenous levels of total DYRK4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
520
Applicable Applications for anti-DYRK4 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm.
Conjugation
Unconjugated
IHC (Immunohistochemisry)
(AAA323526 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry)
(AAA323526 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of DYRK4 expression in NCI-H292 cell line lysates ; developed using the ECL technique, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of DYRK4 expression in NCI-H292 cell line lysates ; developed using the ECL technique, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence)
(AAA323526 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323526 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-DYRK4 antibody
Description: Possible non-essential role in spermiogenesis.
Post-translational Modifications: Autophosphorylation on Tyr-264 in the activation loop is required for kinase activity.
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Post-translational Modifications: Autophosphorylation on Tyr-264 in the activation loop is required for kinase activity.
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 60 kDa
Predicted: 60 kDa
Predicted: 60 kDa
NCBI Official Full Name
dual specificity tyrosine-phosphorylation-regulated kinase 4 isoform 1
NCBI Official Synonym Full Names
dual specificity tyrosine phosphorylation regulated kinase 4
NCBI Official Symbol
DYRK4
NCBI Protein Information
dual specificity tyrosine-phosphorylation-regulated kinase 4
UniProt Protein Name
Dual specificity tyrosine-phosphorylation-regulated kinase 4
UniProt Gene Name
DYRK4
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Product Notes
The DYRK4 dyrk4 (Catalog #AAA323526) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DYRK4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DYRK4 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DYRK4 dyrk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYRK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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