Sheep anti-Human, Rat E1A (adenovirus early region 1) Polyclonal Antibody | anti-E1A antibody
E1A (adenovirus early region 1)
Reactivity
Human, Rat
Applications
Immunoprecipitation
Purity
Ammonium Sulfate Precipitation
Synonyms
E1A (adenovirus early region 1), Antibody; E1A (adenovirus early region 1); E1A Early Adenovirus region 1; anti-E1A antibody
Host
Sheep
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Ammonium Sulfate Precipitation
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Sequence Length
289
Applicable Applications for anti-E1A antibody
IP (Immunoprecipitation)
Immunogen
A GST-EIA fusion protein recognizing a 43kD protein
Positive Control
293 cells are human embryonic kidney cells that have been transformed with the adenovirus genes E1A and E1B, which are required for adenovirus propagation. This cell line can be used as a positive control for this antibody. This antibody is suitable for use with his tagged or gst tagged recombinant proteins.
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Related Product Information for anti-E1A antibody
The immunogen for the anti-EIA was a recombinant E1A protein recognizing a 43kD protein. EIA gene encodes potent onco-proteins that modify the normal transcriptional growth regulation of key cellular genes and thus alter cell cycle control. E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif
Product Categories/Family for anti-E1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,851 Da
NCBI Official Full Name
E1A
UniProt Protein Name
Early E1A 32 kDa protein
UniProt Entry Name
E1A_ADE02
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Product Notes
The E1A (Catalog #AAA60509) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The E1A (adenovirus early region 1) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's E1A (adenovirus early region 1) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation). Researchers should empirically determine the suitability of the E1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E1A (adenovirus early region 1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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