<
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cell. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4 degree overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).)
Rabbit E2F-1 Polyclonal Antibody | anti-E2F-1 antibody
E2F-1 Rabbit Polyclonal Antibody
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
E2F-1, Antibody; E2F-1 Rabbit Polyclonal Antibody; E2F1 RBBP3; BH6842; E2F transcription factor 1; anti-E2F-1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous E2F-1 protein
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-E2F-1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
Synthetic peptide from human protein at AA range: 100-170
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cell. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4 degree overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).)
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cell. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4 degree overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).)
IF (Immunofluorescence)
(Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,E2F-1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,E2F-1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
WB (Western Blot)
(Western Blot analysis of MOUSE-BRAIN cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000)
WB (Western Blot)
(Western Blot analysis of MOUSE-BRAIN cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000)
Related Product Information for anti-E2F-1 antibody
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,920 Da
NCBI Official Full Name
transcription factor E2F1
NCBI Official Synonym Full Names
E2F transcription factor 1
NCBI Official Symbol
E2F1
NCBI Official Synonym Symbols
RBP3; E2F-1; RBAP1; RBBP3
NCBI Protein Information
transcription factor E2F1; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1
UniProt Protein Name
Transcription factor E2F1
UniProt Gene Name
E2F1
UniProt Synonym Gene Names
RBBP3; E2F-1; RBAP-1; RBBP-3
UniProt Entry Name
E2F1_HUMAN
Similar Products
Product Notes
The E2F-1 e2f1 (Catalog #AAA319025) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The E2F-1 Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's E2F-1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the E2F-1 e2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.