WB (Western Blot)
(E2F2 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the E2F2 antibody detected the E2F2 protein (arrow).)
Rabbit anti-Human, mouse E2F2 Polyclonal Antibody | anti-E2F2 antibody
E2F2 Antibody (Center)
Gene Names
E2F2; E2F-2
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
E2F2, Antibody; E2F2 Antibody (Center); Transcription factor E2F2; E2F-2; E2F2; anti-E2F2 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This E2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the Central region of human E2F2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
258-286
Sequence Length
437
Applicable Applications for anti-E2F2 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(E2F2 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the E2F2 antibody detected the E2F2 protein (arrow).)
WB (Western Blot)
(E2F2 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the E2F2 antibody detected the E2F2 protein (arrow).)
WB (Western Blot)
(All lanes : Anti-E2F2 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-E2F2 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-E2F2 antibody
E2F2 is a member of the E2F
family of transcription factors. The E2F family plays a crucial
role in the control of cell cycle and action of tumor suppressor
proteins and is also a target of the transforming proteins of small
DNA tumor viruses. The E2F proteins contain several evolutionally
conserved domains found in most members of the family. These
domains include a DNA binding domain, a dimerization domain which
determines interaction with the differentiation regulated
transcription factor proteins (DP), a transactivation domain
enriched in acidic amino acids, and a tumor suppressor protein
association domain which is embedded within the transactivation
domain. This protein and another 2 members, E2F1 and E2F3, have an
additional cyclin binding domain. This protein binds specifically
to retinoblastoma protein pRB in a cell-cycle dependent manner, and
it exhibits overall 46% amino acid identity to E2F1. [provided by
RefSeq].
family of transcription factors. The E2F family plays a crucial
role in the control of cell cycle and action of tumor suppressor
proteins and is also a target of the transforming proteins of small
DNA tumor viruses. The E2F proteins contain several evolutionally
conserved domains found in most members of the family. These
domains include a DNA binding domain, a dimerization domain which
determines interaction with the differentiation regulated
transcription factor proteins (DP), a transactivation domain
enriched in acidic amino acids, and a tumor suppressor protein
association domain which is embedded within the transactivation
domain. This protein and another 2 members, E2F1 and E2F3, have an
additional cyclin binding domain. This protein binds specifically
to retinoblastoma protein pRB in a cell-cycle dependent manner, and
it exhibits overall 46% amino acid identity to E2F1. [provided by
RefSeq].
References
Revenko, A.S., et al. Mol. Cell. Biol. 30(22):5260-5272(2010)
Hayami, S., et al. Mol. Cancer 9, 59 (2010) :
Chen, J., et al. Cancer Causes Control 20(9):1769-1777(2009)
Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
Lal, A., et al. Mol. Cell 35(5):610-625(2009)
Hayami, S., et al. Mol. Cancer 9, 59 (2010) :
Chen, J., et al. Cancer Causes Control 20(9):1769-1777(2009)
Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
Lal, A., et al. Mol. Cell 35(5):610-625(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47506
NCBI Official Full Name
transcription factor E2F2
NCBI Official Synonym Full Names
E2F transcription factor 2
NCBI Official Symbol
E2F2
NCBI Official Synonym Symbols
E2F-2
NCBI Protein Information
transcription factor E2F2
UniProt Protein Name
Transcription factor E2F2
UniProt Gene Name
E2F2
UniProt Synonym Gene Names
E2F-2
UniProt Entry Name
E2F2_HUMAN
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Product Notes
The E2F2 e2f2 (Catalog #AAA285024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 258-286. The E2F2 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's E2F2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the E2F2 e2f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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