IF (Immunofluorescence)
(Immunofluorescence of E2F3 in human lymph node tissue with E2F3 antibody at 20 μg/ml.)
Rabbit anti-Human, Mouse E2F3 Polyclonal Antibody | anti-E2F3 antibody
E2F3 Antibody
Gene Names
E2F3; E2F-3
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
E2F3 antibody is affinity chromatography purified via peptide column.
Synonyms
E2F3, Antibody; E2F3 Antibody; E2F transcription factor 3; E2F-3; anti-E2F3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.
Purity/Purification
E2F3 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
465
Applicable Applications for anti-E2F3 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3.
Buffer
E2F3 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
E2F3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of E2F3 in human lymph node tissue with E2F3 antibody at 20 μg/ml.)
IF (Immunofluorescence)
(Immunofluorescence of E2F3 in human lymph node tissue with E2F3 antibody at 20 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of E2F3 in human lymph node tissue with E2F3 antibody at 5 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of E2F3 in human lymph node tissue with E2F3 antibody at 5 μg/ml.)
WB (Western Blot)
(Western blot analysis of E2F3 in human lymph node tissue lysate with E2F3 antibody at 1 μg/ml.)
WB (Western Blot)
(Western blot analysis of E2F3 in human lymph node tissue lysate with E2F3 antibody at 1 μg/ml.)
Related Product Information for anti-E2F3 antibody
The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 51 kDa
Observed: 56 kDa
Observed: 56 kDa
NCBI Official Full Name
transcription factor E2F3 isoform 1
NCBI Official Synonym Full Names
E2F transcription factor 3
NCBI Official Symbol
E2F3
NCBI Official Synonym Symbols
E2F-3
NCBI Protein Information
transcription factor E2F3
UniProt Protein Name
Transcription factor E2F3
UniProt Gene Name
E2F3
UniProt Synonym Gene Names
KIAA0075; E2F-3
UniProt Entry Name
E2F3_HUMAN
Similar Products
Product Notes
The E2F3 e2f3 (Catalog #AAA40562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The E2F3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's E2F3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the E2F3 e2f3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.