IP (Immunoprecipitation)
(Detection of human EAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EAF2 antibody AAA211901 used for IP at 3 ug/mg lysate. EAF2 was also immunoprecipitated by rabbit anti-EAF2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated EAF2, AAA211901 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.)
Rabbit anti-Human EAF2 Polyclonal Antibody | anti-EAF2 antibody
Rabbit anti-EAF2 Antibody, Affinity Purified
Gene Names
EAF2; U19; BM040; TRAITS
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
EAF2, Antibody; Rabbit anti-EAF2 Antibody, Affinity Purified; BM040; ELL-associated factor 2; testosterone-regulated apoptosis inducer and tumor suppressor protein; TRAITS; U19; ELL associated factor 2; anti-EAF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-EAF2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 210 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human EAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EAF2 antibody AAA211901 used for IP at 3 ug/mg lysate. EAF2 was also immunoprecipitated by rabbit anti-EAF2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated EAF2, AAA211901 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.)
IP (Immunoprecipitation)
(Detection of human EAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EAF2 antibody AAA211901 used for IP at 3 ug/mg lysate. EAF2 was also immunoprecipitated by rabbit anti-EAF2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated EAF2, AAA211901 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human EAF2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-EAF2 (Cat. No. AAA211901) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human EAF2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-EAF2 (Cat. No. AAA211901) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-EAF2 antibody
ELL-associated factor 2 (EAF2) acts as a transcriptional transactivator of TCEA1 elongation activity. It is also a transcriptional transactivator of ELL (eleven nineteen lysine-rich leukemia) 1 and ELL2 elongation activities. EAF2 has been shown to be a potent inducer of apoptosis in prostatic and non-prostatic cell lines and to inhibit prostate tumor growth in vivo [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96CJ1].
Product Categories/Family for anti-EAF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,091 Da
NCBI Official Full Name
ELL-associated factor 2 isoform 1
NCBI Official Synonym Full Names
ELL associated factor 2
NCBI Official Symbol
EAF2
NCBI Official Synonym Symbols
U19; BM040; TRAITS
NCBI Protein Information
ELL-associated factor 2
UniProt Protein Name
ELL-associated factor 2
UniProt Gene Name
EAF2
UniProt Synonym Gene Names
TRAITS
UniProt Entry Name
EAF2_HUMAN
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Product Notes
The EAF2 eaf2 (Catalog #AAA211901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EAF2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EAF2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the EAF2 eaf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EAF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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