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product-image-AAA286031_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Rabbit anti-Human EARS2 Polyclonal Antibody | anti-EARS2 antibody

EARS2 Antibody (C-term)

Gene Names
EARS2; MSE1; gluRS; COXPD12
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EARS2, Antibody; EARS2 Antibody (C-term); Probable glutamate--tRNA ligase; mitochondrial; Glutamyl-tRNA synthetase; GluRS; EARS2; KIAA1970; anti-EARS2 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-523 amino acids from the C-terminal region of human EARS2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
495-523
Sequence Length
523
Applicable Applications for anti-EARS2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

product-image-AAA286031_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab.)

product-image-AAA286031_WB15.jpg WB (Western Blot) (Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-EARS2 antibody
Glutamyl-tRNA synthetase (GluRS or EARS2) a class I aminoacyl-tRNA synthetase (aaRS), is primarily responsible for the glutamylation of tRNAGlu. It is part of the ?minimal set?of seventeen aaRSs found in every living organism and its presence is essential for the viability of cells.
Product Categories/Family for anti-EARS2 antibody
References
Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58689
NCBI Official Full Name
probable glutamate--tRNA ligase, mitochondrial isoform 1
NCBI Official Synonym Full Names
glutamyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
EARS2
NCBI Official Synonym Symbols
MSE1; gluRS; COXPD12
NCBI Protein Information
probable glutamate--tRNA ligase, mitochondrial
UniProt Protein Name
Probable glutamate--tRNA ligase, mitochondrial
UniProt Gene Name
EARS2
UniProt Synonym Gene Names
KIAA1970; GluRS
UniProt Entry Name
SYEM_HUMAN

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Product Notes

The EARS2 ears2 (Catalog #AAA286031) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 495-523. The EARS2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EARS2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EARS2 ears2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EARS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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