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IHC (Immunohistochemistry)
(AAA329030 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit ECM2 Polyclonal Antibody | anti-ECM2 antibody
ECM2 Antibody
Reactivity
Human, Mouse, RatPredicted Reactivity: Bovine(88%), Horse(100%), Sheep(88%), Dog(100%), Chicken(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
ECM2, Antibody; ECM2 Antibody; Ecm2; ECM2_HUMAN; Extracellular matrix protein 2; Matrix glycoprotein SC1/ECM2; MGC126355; MGC126356; anti-ECM2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine(88%), Horse(100%), Sheep(88%), Dog(100%), Chicken(86%)
Predicted Reactivity: Bovine(88%), Horse(100%), Sheep(88%), Dog(100%), Chicken(86%)
Clonality
Polyclonal
Isotype
IgG
Specificity
ECM2 Antibody detects endogenous levels of total ECM2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
677
Applicable Applications for anti-ECM2 antibody
ELISA
Immunogen
A synthesized peptide derived from human ECM2, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA329030 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA329030 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA329030 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA329030 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329030 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329030 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Human cerebellum, using ECM2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Human cerebellum, using ECM2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-ECM2 antibody
Function: Promotes matrix assembly and cell adhesiveness.
Subcellular Location: Secreted>Extracellular space>Extracellular matrix.
Tissue Specificity: Expressed predominantly in adipose tissue as well as female-specific organs such as mammary gland, ovary, and uterus.
Subunit Structure: Interacts with numerous extracellular matrix proteins (By similarity). Interacts with MSL1 and RASSF1 (By similarity).
Similarity: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Subcellular Location: Secreted>Extracellular space>Extracellular matrix.
Tissue Specificity: Expressed predominantly in adipose tissue as well as female-specific organs such as mammary gland, ovary, and uterus.
Subunit Structure: Interacts with numerous extracellular matrix proteins (By similarity). Interacts with MSL1 and RASSF1 (By similarity).
Similarity: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)79kD.
Predicted Molecular Weight: (Calculated)80kDa.
Predicted Molecular Weight: (Calculated)80kDa.
NCBI Official Full Name
extracellular matrix protein 2 isoform 2
NCBI Official Synonym Full Names
extracellular matrix protein 2
NCBI Official Symbol
ECM2
NCBI Protein Information
extracellular matrix protein 2
UniProt Protein Name
Extracellular matrix protein 2
UniProt Gene Name
ECM2
UniProt Entry Name
ECM2_HUMAN
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Product Notes
The ECM2 ecm2 (Catalog #AAA329030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ECM2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Bovine(88%), Horse(100%), Sheep(88%), Dog(100%), Chicken(86%) and may cross-react with other species as described in the data sheet. AAA Biotech's ECM2 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the ECM2 ecm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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