IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit EDG4 Polyclonal Antibody | anti-LPAR2 antibody
EDG4 Antibody (N-term)
Gene Names
LPAR2; EDG4; LPA2; EDG-4; LPA-2
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Immunohistochemistry, ELISA, Western Blot
Synonyms
EDG4, Antibody; EDG4 Antibody (N-term); Lysophosphatidic acid receptor 2; LPA receptor 2; LPA-2; Lysophosphatidic acid receptor Edg-4; LPAR2; EDG4; LPA2; anti-LPAR2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EDG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-32 amino acids from the N-terminal region of human EDG4.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
3-32
Sequence Length
351
Applicable Applications for anti-LPAR2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Stimulates phospholipase C (PLC) activity in a manner that is independent of RALA activation.
Cellular Location
Cell surface. Cell membrane; Multi-pass membrane protein. Note=Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA colocalizes with RALA in the endocytic vesicles.
Tissue Location
Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate. Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon.
Antigen Region
3-32
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks.
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(The anti-EDG4 N-term Antibody is used in Western blot to detect EDG4 in HL60 lysate.)
WB (Western Blot)
(The anti-EDG4 N-term Antibody is used in Western blot to detect EDG4 in HL60 lysate.)
WB (Western Blot)
(Western blot analysis of lysate from MDA-MB-231 cell line, using EDG4 Antibody (N18). AAA284522 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from MDA-MB-231 cell line, using EDG4 Antibody (N18). AAA284522 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-LPAR2 antibody
EDG4 is a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins.
Product Categories/Family for anti-LPAR2 antibody
References
Hu, Y.L., et al., J. Natl. Cancer Inst. 95(10):733-740 (2003).
Fujita, T., et al., Cancer Lett. 192(2):161-169 (2003).
Contos, J.J., et al., Genomics 64(2):155-169 (2000).
Zheng, Y., et al., FASEB J. 14(15):2387-2389 (2000).
Young, K.W., et al., J. Biol. Chem. 275(49):38532-38539 (2000).
Fujita, T., et al., Cancer Lett. 192(2):161-169 (2003).
Contos, J.J., et al., Genomics 64(2):155-169 (2000).
Zheng, Y., et al., FASEB J. 14(15):2387-2389 (2000).
Young, K.W., et al., J. Biol. Chem. 275(49):38532-38539 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
lysophosphatidic acid receptor 2
NCBI Official Synonym Full Names
lysophosphatidic acid receptor 2
NCBI Official Symbol
LPAR2
NCBI Official Synonym Symbols
EDG4; LPA2; EDG-4; LPA-2
NCBI Protein Information
lysophosphatidic acid receptor 2
UniProt Protein Name
Lysophosphatidic acid receptor 2
UniProt Gene Name
LPAR2
UniProt Synonym Gene Names
EDG4; LPA2; LPA receptor 2; LPA-2
UniProt Entry Name
LPAR2_HUMAN
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Product Notes
The LPAR2 lpar2 (Catalog #AAA284522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 3-32. The EDG4 Antibody (N-term) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's EDG4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LPAR2 lpar2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EDG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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