FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit EDNRA Polyclonal Antibody | anti-EDNRA antibody
EDNRA Antibody (Center)
Gene Names
EDNRA; ETA; ET-A; ETAR; ETRA; MFDA; ETA-R; hET-AR
Reactivity
HumanPredicted Species: Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EDNRA, Antibody; EDNRA Antibody (Center); Endothelin-1 receptor; Endothelin A receptor; ET-A; ETA-R; hET-AR; EDNRA; ETA; ETRA; anti-EDNRA antibody
Host
Rabbit
Reactivity
Human
Predicted Species: Mouse, Rat
Predicted Species: Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This EDNRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human EDNRA.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
125-154
Applicable Applications for anti-EDNRA antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence)
Function
Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
Cellular Location
Cell membrane; Multi-pass membrane protein.
Tissue Location
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of EDNRA antibody (Center) (Cat.# AAA285072) in CEM cell line lysates (35ug/lane). EDNRA (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of EDNRA antibody (Center) (Cat.# AAA285072) in CEM cell line lysates (35ug/lane). EDNRA (arrow) was detected using the purified Pab.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EDNRA Antibody (Center)(AAA285072) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EDNRA Antibody (Center)(AAA285072) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-EDNRA antibody
EDNRA is a receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Product Categories/Family for anti-EDNRA antibody
References
Cardillo,C., Arthritis Rheum. 60 (6), 1840-1844 (2009) Hayzer,D.J., Am. J. Med. Sci. 304 (4), 231-238 (1992)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48722
NCBI Official Full Name
endothelin-1 receptor isoform b
NCBI Official Synonym Full Names
endothelin receptor type A
NCBI Official Symbol
EDNRA
NCBI Official Synonym Symbols
ETA; ET-A; ETAR; ETRA; MFDA; ETA-R; hET-AR
NCBI Protein Information
endothelin-1 receptor
UniProt Protein Name
Endothelin-1 receptor
UniProt Gene Name
EDNRA
UniProt Synonym Gene Names
ETA; ETRA; ET-A; ETA-R; hET-AR
UniProt Entry Name
EDNRA_HUMAN
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Product Notes
The EDNRA ednra (Catalog #AAA285072) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 125-154. The EDNRA Antibody (Center) reacts with Human Predicted Species: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EDNRA can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the EDNRA ednra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EDNRA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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