IP (Immunoprecipitation)
(EEF1B2 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-EEF1B2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EEF1B2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size: 32 kDa)
Rabbit anti-Human EEF1B2 Polyclonal Antibody | anti-EEF1B2 antibody
Anti-EEF1B2 Antibody, Rabbit Polyclonal
Gene Names
EEF1B2; EF1B; EEF1B; EEF1B1
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
EEF1B2, Antibody; Anti-EEF1B2 Antibody, Rabbit Polyclonal; EF1B/EEF1B2 Antibody, Rabbit PAb, Antigen Affinity Purified; eukaryotic translation elongation factor 1 beta 2; Anti-EEF1B Antibody; Anti-EEF1B1 Antibody; Anti-EF1B Antibody; anti-EEF1B2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human EF1B/EEF1B2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-EEF1B2 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human EF1B/EEF1B2 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human EF1B/EEF1B2 (rh EF1B/EEF1B2). EF1B/EEF1B2 specific IgG was purified by Human EF1B/EEF1B2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(EEF1B2 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-EEF1B2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EEF1B2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size: 32 kDa)
IP (Immunoprecipitation)
(EEF1B2 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-EEF1B2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EEF1B2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size: 32 kDa)
WB (Western Blot)
(Anti-EEF1B2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:32 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-EEF1B2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:32 kDa(We are unsure as to the identity of these extra bands.))
IHC (Immunohistochemistry)
(Immunochemical staining of human EEF1B2 in human breast carcinoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human EEF1B2 in human breast carcinoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-EEF1B2 antibody
EF1B, also known as EEF1B2, is a translation elongation factor. It belongs to the EF-1-beta/EF-1-delta family. Elongation factors are a set of proteins that are used in protein synthesis in the cell. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. EF1B is more complex in eukaryotes than in bacteria, and consists of three subunits: EF1B-alpha, EF1B-gamma and EF1B-beta. EF1B contains 1 GST C-terminal domain. It is involved in the transfer of aminoacylated tRNAs to the ribosome. EF1B is required to regenerate EF1A from its inactive form (EF1A-GDP) to its active form (EF1A-GTP). EF1A is then ready to interact with a new aminoacyl-tRNA to begin the cycle again.
References
Pizzuti A. et al., 1994, Biochem Biophys Res Commun. 197 (1): 154-62. Rual. et al., 2005, Nature. 437 (7062): 1173-8. Stelzl. et al., 2005, Cell. 122 (6): 957-68. Sang Lee. et al., 2002, Biochem Biophys Res Commun. 291 (1): 158-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,764 Da
NCBI Official Full Name
elongation factor 1-beta
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 beta 2
NCBI Official Symbol
EEF1B2
NCBI Official Synonym Symbols
EF1B; EEF1B; EEF1B1
NCBI Protein Information
elongation factor 1-beta; EF-1-beta; eukaryotic translation elongation factor 1 beta 1
UniProt Protein Name
Elongation factor 1-beta
UniProt Gene Name
EEF1B2
UniProt Synonym Gene Names
EEF1B; EF1B; EF-1-beta
UniProt Entry Name
EF1B_HUMAN
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Product Notes
The EEF1B2 eef1b2 (Catalog #AAA255778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EEF1B2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1B2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EEF1B2 eef1b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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