IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EFNB2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human EFNB2 Polyclonal Antibody | anti-EFNB2 antibody
EFNB2 Antibody (Center)
Gene Names
EFNB2; HTKL; EPLG5; Htk-L; LERK5
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EFNB2, Antibody; EFNB2 Antibody (Center); Ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L; EFNB2; EPLG5; HTKL; LERK5; anti-EFNB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EFNB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-186 amino acids from the Central region of human EFNB2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.47 (varies by lot)
Sequence Positions
157-186
Sequence Length
333
Applicable Applications for anti-EFNB2 antibody
IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EFNB2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EFNB2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(EFNB2 Antibody (Center) western blot analysis in Hela,293 cell line lysates (35ug/lane).This demonstrates the EFNB2 antibody detected the EFNB2 protein (arrow).)
WB (Western Blot)
(EFNB2 Antibody (Center) western blot analysis in Hela,293 cell line lysates (35ug/lane).This demonstrates the EFNB2 antibody detected the EFNB2 protein (arrow).)
IHC (Immunohistochemistry)
(EFNB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EFNB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(EFNB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EFNB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-EFNB2 antibody
This gene encodes a member of the ephrin (EPH) family. The
ephrins and EPH-related receptors comprise the largest subfamily of
receptor protein-tyrosine kinases and have been implicated in
mediating developmental events, especially in the nervous system
and in erythropoiesis. Based on their structures and sequence
relationships, ephrins are divided into the ephrin-A (EFNA) class,
which are anchored to the membrane by a
glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB)
class, which are transmembrane proteins. This gene encodes an EFNB
class ephrin which binds to the EPHB4 and EPHA3 receptors.
ephrins and EPH-related receptors comprise the largest subfamily of
receptor protein-tyrosine kinases and have been implicated in
mediating developmental events, especially in the nervous system
and in erythropoiesis. Based on their structures and sequence
relationships, ephrins are divided into the ephrin-A (EFNA) class,
which are anchored to the membrane by a
glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB)
class, which are transmembrane proteins. This gene encodes an EFNB
class ephrin which binds to the EPHB4 and EPHA3 receptors.
Product Categories/Family for anti-EFNB2 antibody
References
Zhang, R., et al. Psychiatry Res 180(1):5-9(2010)
Bochenek, M.L., et al. J. Cell. Sci. 123 (PT 8), 1235-1246 (2010) :
Nakada, M., et al. Int. J. Cancer 126(5):1155-1165(2010)
Qin, H., et al. J. Biol. Chem. 285(1):644-654(2010)
Kwan Tat, S., et al. Arthritis Res. Ther. 11 (4), R119 (2009) :
Bochenek, M.L., et al. J. Cell. Sci. 123 (PT 8), 1235-1246 (2010) :
Nakada, M., et al. Int. J. Cancer 126(5):1155-1165(2010)
Qin, H., et al. J. Biol. Chem. 285(1):644-654(2010)
Kwan Tat, S., et al. Arthritis Res. Ther. 11 (4), R119 (2009) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36923
NCBI Official Full Name
ephrin-B2
NCBI Official Synonym Full Names
ephrin-B2
NCBI Official Symbol
EFNB2
NCBI Official Synonym Symbols
HTKL; EPLG5; Htk-L; LERK5
NCBI Protein Information
ephrin-B2
UniProt Protein Name
Ephrin-B2
UniProt Gene Name
EFNB2
UniProt Synonym Gene Names
EPLG5; HTKL; LERK5; LERK-5; HTK-L
UniProt Entry Name
EFNB2_HUMAN
Similar Products
Product Notes
The EFNB2 efnb2 (Catalog #AAA290020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 157-186. The EFNB2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EFNB2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the EFNB2 efnb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EFNB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.