Application Data
(Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (AAA49963) following emzyme mediated antigen retrieval using proteinase K)
Rabbit EGF Polyclonal Antibody | anti-EGF antibody
RABBIT ANTI HUMAN EGF
Gene Names
EGF; URG; HOMG4
Applications
Western Blot, Immunohistochemistry, Functional Assay, ELISA
Synonyms
EGF, Antibody; RABBIT ANTI HUMAN EGF; anti-EGF antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Form/Format
Purified
Purified IgG - lyophilised
Purified IgG - lyophilised
Concentration
IgG concentration 1.0mg/ml after reconstitution (varies by lot)
Sequence Length
1166
Applicable Applications for anti-EGF antibody
WB (Western Blot), IHC (Immunohistochemistry), FA (Functional Assay), ELISA
Reconstitution
Reconstitute with 0.1ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add azide.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AAA Biotech recommends that the vial is gently mixed after reconstitution.
Immunogen
Recombinant human EGF.
Histology Positive Control Tissue
Histology Positive Control Tissue
Antiserum Preparation
Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Application Data
(Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (AAA49963) following emzyme mediated antigen retrieval using proteinase K)
Application Data
(Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (AAA49963) following emzyme mediated antigen retrieval using proteinase K)
Application Data
(Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (AAA49963) following emzyme mediated antigen retrieval using proteinase K)
Application Data
(Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (AAA49963) following emzyme mediated antigen retrieval using proteinase K)
Related Product Information for anti-EGF antibody
Rabbit anti human EGF recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, which is expressed by a variety of cells.. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the 170kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a, VGF (vaccinia virus growth factor) and Betacellulin, produced by certain tumour cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
129,746 Da
NCBI Official Full Name
pro-epidermal growth factor isoform 2 preproprotein
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
EGF
NCBI Official Synonym Symbols
URG; HOMG4
NCBI Protein Information
pro-epidermal growth factor; beta-urogastrone
UniProt Protein Name
Pro-epidermal growth factor
UniProt Gene Name
EGF
UniProt Synonym Gene Names
EGF
UniProt Entry Name
EGF_HUMAN
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Product Notes
The EGF egf (Catalog #AAA49963) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EGF can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), FA (Functional Assay), ELISA. Researchers should empirically determine the suitability of the EGF egf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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