Rabbit EGFR Polyclonal Antibody | anti-EGFR antibody

EGFR Antibody (N-term)

Cat. Num. AAA287827

• Gene Names: EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• Reactivity: Human (Predicted Reactivity: Chicken, Mouse)
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: EGFR, Antibody; EGFR Antibody (N-term); Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB; ERBB1; HER1; anti-EGFR antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Chicken, Mouse)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the N-terminal region of human EGFR.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 259-287
• Sequence Length: 628

• Applicable Applications for anti-EGFR antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Function: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin- binding EGF (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed: ). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed: ). Plays a role in enhancing learning and memory performance (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysates from A431, HUVEC cell line (from left to right), using EGFR Antibody (N-term). AAA287827 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H. skin section using EGFR Antibody (N-term). AAA287827 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Related Product Information for anti-EGFR antibody

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Product Categories/Family for anti-EGFR antibody

Crown Antibodies; Cancer; Signal Transduction

References

Abdallah, R.T., et al. J. Biol. Chem. 285(45):35206-35215(2010)
Lu, C., et al. Mol. Cell. Biol. 30(22):5432-5443(2010)
Rosell, R., et al. Ann. N. Y. Acad. Sci. 1210, 45-52 (2010) :
Hata, A., et al. J Thorac Oncol 5(10):1524-1528(2010)
Aguirre, A., et al. Nature 467(7313):323-327(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 29725609
• NCBI GeneID: 1956
• NCBI Accession #: NP_005219.2; NP_958439.1; NP_958440.1; NP_958441.1
• NCBI GenBank Nucleotide #: NM_005228.3
• UniProt Accession #: P00533; O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7
• NCBI Official Full Name: epidermal growth factor receptor isoform a
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: ERBB; ERBB1; HER1
• UniProt Entry Name: EGFR_HUMAN